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Next Generation Sequencing Facilitates Quantitative Analysis of Osteoarthritis related long non-coding RNAs (LcnRNAs)

ABSTRACT: Purpose: LncRNAs are important moleculars in immune responses. The goals of this study are to identify osteoarthritis related lncRNAs. Methods: PBMCs from healthy control and osteoarthritis patients were collected. Then, the next-generation libraries of mRNA were prepared using VAHTS mRNA-seq v2 Library Prep Kit for Illumina® (Vazyme, Nanjing, China). The Library quality was determined by Bioanalyzer 4200 (Agilent, Santa Clara, CA, USA). Then the mRNA-seq libraries were sequenced in HiSeq ⅹ10 system (Illumina, San Diego, CA, USA) on a 150bp paired-end run. The differentially expressed lncRNAs were selected as having more than 1 fold difference in their geometrical mean expression between the compared groups and a statistically significant p-value (<0.05) by analysis of DEseq2. The GO analysis on differentially expressed lncRNAs was performed with an R package: Clusterprofiler using a p<0.05 to define statistically enriched GO categories. Results: 18 lncRNAs decreased significantly in osteoarthritis patients.

ORGANISM(S): Homo sapiens

PROVIDER: GSE163552 | GEO | 2021/02/08

REPOSITORIES: GEO

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