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MITF reprograms the extracellular matrix and focal adhesion in melanoma

ABSTRACT: To assess the effects of permanent loss of MITF in melanoma cells, we used the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 technique to generate MITF knockout (KO) cell lines in the human hypo-tetraploid SkMel28 melanoma cell line (containing four copies of MITF). We targeted exon 6 (containing the DNA binding domain) of MITF the resulting isogenic cell line is hereafter referred to as ΔMITF-X6. We also performed siRNA mediated transient knock down of MITF along with control siCTRL in 501Mel cells. Furthermore, we used a stable doxycycline inducible over-expression cell line containing FLAG tagged MITF and a control cells with empty vector FLAG in A375P. From our RNA-sequencing studies we found that MITF plays a critical role as a repressor of extracellular matrix gene expression and is actively involved in shaping the microenvironment of melanoma cells in a cell-autonomous manner.

ORGANISM(S): Homo sapiens

PROVIDER: GSE163646 | GEO | 2020/12/22

REPOSITORIES: GEO

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Essential role of microphthalmia transcription factor for DNA replication, mitosis and genomic stability in melanoma

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IGR_MITF_STUDY_BBRB

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Homo sapiens

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| PRJNA289013 | ENA

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