Differential expression of genes on the Enterococcus faecalis plasmid pCF10 in the presence and absence of prgU

ABSTRACT: Efficient horizontal gene transfer of the conjugative plasmid pCF10 from Enterococcus faecalis depends on the expression of its Type 4 Secretion System (T4SS) genes, controlled by the PQ promoter. The PQ promoter is strictly regulated, partially to limit cell toxicity caused by overproduction of PrgB, a T4SS adhesin. PrgU plays an important role in regulating this toxicity by decreasing PrgB levels. PrgU has an RNA-binding fold, prompting us to test whether PrgU exerts its regulatory control through binding of prgQ transcripts. We used a combination of in vivo methods to quantify PrgU effects on prgQ transcripts at both single cell and population levels.

ORGANISM(S): Enterococcus faecalis

PROVIDER: GSE163794 | GEO | 2020/12/24

REPOSITORIES: GEO