Transcriptomics

Dataset Information

0

Gene expression analyses of C/EBPb knockout in stem/progenitor cell populations


ABSTRACT: CCAAT/enhancer binding protein beta (C/EBPb) is a member of a family of highly conserved transcription factors that regulates numerous genes involved in proliferation and differentiation in a variety of tissues. C/EBPb is deregulated in human breast cancer and germline deletion of this gene results in multiple defects in mammary gland development. We hypothesized that C/EBPb regulates mammary stem cell self-renewal, maintenance and/or differentiation through the regulation of multiple target genes that coordinate mammary gland development. Utilizing both a germline knockout mouse model and a conditional knockout strategy, we demonstrated that mammosphere formation was significantly decreased in C/EBPb-deficient mammary epithelial cells (MECs). The ability of C/EBPb-deleted MECs to regenerate the mammary gland in vivo was severely impaired when transplanted at limiting dilution. Furthermore, serial transplantation of C/EBPb-null mammary tissue resulted in decreased outgrowth potential when compared to wildtype, and an early senescence phenotype. Flow cytometric analysis revealed that C/EBPb-null MECs contain a lower frequency of repopulating stem cells accompanied by an increase in committed, differentiated luminal cells as compared to wildtype. Microarray analysis of stem/progenitor cell populations was performed and revealed an alteration in cell fate specification in C/EBPb-null glands, exemplified by the aberrant expression of basal markers in the luminal cell compartment. Collectively, our studies demonstrate that C/EBPb is a critical regulator of mammary stem cell differentiation, and an important determinant of luminal cell fate specification. Keywords: multiple group comparison

ORGANISM(S): Mus musculus

PROVIDER: GSE16380 | GEO | 2010/01/28

SECONDARY ACCESSION(S): PRJNA115291

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2010-02-05 | E-GEOD-16380 | biostudies-arrayexpress
2013-09-05 | E-GEOD-45826 | biostudies-arrayexpress
2020-08-01 | GSE149275 | GEO
2024-07-10 | GSE252083 | GEO
2010-07-01 | E-GEOD-5602 | biostudies-arrayexpress
2024-06-11 | GSE207893 | GEO
2022-12-20 | GSE142659 | GEO
2022-12-20 | GSE142550 | GEO
2022-12-20 | GSE142549 | GEO
2022-12-20 | GSE142548 | GEO