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Transcriptomic analysis of the duckling liver mRNA methylome following infection by the virulent and attenuated Hepatitis A virus type 1 strains

ABSTRACT: Duck hepatitis A virus (DHAV) modulates host physiology and gene expression. The RNA modiﬁcation N6-methyladenosine (m6A) modulates mRNA fate and thus affects many biological processes in mammals. Neither modifications on mRNAs have been reported in avian species. Here, we used immunoblot analysis and LC-MS/MS to show the presence of m6A modification in ducking liver, and a decreased m6A modification by virulent DHAV-1 (CH) strain when compared to the attenuated vaccine DHAV-1 (CK). The m6A-seq analysis of the duckling liver at four post-infection time points revealed predominant distribution at CDS region, and a virus-induced time-dependent increase to decrease change in the peak signals. In particular, we only found ACU consensus sequence in the motif enriched by the duckling m6A peaks, which is a part of the classic mammalian m6A motif GGACU. Notably, the most enriched motif in the duckling m6A peaks was GAAGAAG. Strikingly, combination analysis of the difference in the levels of gene expression and m6A modification in duckling livers infected by CK- and CH-strains showed a strong correlation between these two molecular mechanisms. GO analysis showed that genes showing coupled change in m6A modification and gene expression level in responding to the DHAV-1 virulence were enriched in pathways including defense response to virus and immune response. Overall, this work reveals a sophisticated m6A modification profile in duckling liver, which is sensitive to DHAV-1 infection and involved in distinguishing and responding to the virulence

ORGANISM(S): Anas platyrhynchos

PROVIDER: GSE163905 | GEO | 2022/02/08

REPOSITORIES: GEO

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