Transcriptomics

Dataset Information

0

Cell-Specific Gene Expression in Langerhans Cell Histiocytosis


ABSTRACT: Langerhans-cell histiocytosis (LCH) is characterized by heterogeneous lesions containing CD207+ Langerhans cells (LCs) and lymphocytes. In this study, we isolated CD207+ cells and CD3+ T cells from LCH lesions to determine cell-specific gene expression. Compared to control epidermal CD207+ cells, the LCH CD207+ cells yielded 2113 differentially-expressed genes (FDR<0.01). Surprisingly, expression of many genes previously associated with LCH, including cell-cycle regulators, pro-inflammatory cytokines and chemokines were not significantly different from control LCs in our study. However, several novel genes whose products activate and recruit T cells to sites of inflammation, including SPP1 (osteopontin), were highly over-expressed in LCH CD207+ cells. Furthermore, several genes associated with immature myeloid dendritic cells were over-expressed in LCH CD207+ cells. Compared to the peripheral CD3+ cells from LCH patients, the LCH lesion CD3+ cells yielded only 162 differentially-regulated genes (FDR<0.01), and the expression profile of the LCH lesion CD3+ cells was consistent with an activated regulatory T cell phenotype with increased expression of FOXP3, CTLA4 as well as SPP1. Based on these results, we propose a new model of LCH pathogenesis in which lesions do not arise from epidermal Langerhans cells, but from accumulation of bone-marrow derived immature myeloid dendritic cells that recruit activated lymphocytes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE16395 | GEO | 2010/03/15

SECONDARY ACCESSION(S): PRJNA116283

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2010-03-23 | E-GEOD-16395 | biostudies-arrayexpress
2020-01-21 | GSE122476 | GEO
2020-01-21 | GSE122674 | GEO
2012-10-24 | E-GEOD-35340 | biostudies-arrayexpress
2017-12-20 | GSE108251 | GEO
2012-10-24 | GSE35340 | GEO
2024-12-05 | GSE270891 | GEO
| PRJNA505993 | ENA
| PRJNA505325 | ENA
2013-10-18 | E-GEOD-49085 | biostudies-arrayexpress