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Next Generation Sequencing Facilitates Quantitative Analysis of watermelon fruit Transcriptomes

ABSTRACT: Analysis of gene expression level. The hypothesis tested in the present study was that 5-azacytidine affects fruit ripening.

ORGANISM(S): Citrullus lanatus

PROVIDER: GSE164242 | GEO | 2022/11/15

REPOSITORIES: GEO

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Next Generation Sequencing Facilitates Quantitative Analysis of watermelon fruit Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of watermelon fruit Transcriptomes

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Next Generation Sequencing Facilitates Quantitative Analysis of floral-fruit Transcriptomes

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Project description:Here, we used RNA sequencing and tandem mass tag (TMT)–based quantitative proteomics technology to study the comprehensive mRNA and protein expression changes during fruit development and ripening in watermelon. A total of 6,226 proteins were quantified, and the number of quantitative proteins is the largest in fruit proteome to date, comparable to studies in model organisms such as rice and Arabidopsis. Omics analysis showed that smaller changes occurred in protein abundance compared to mRNA abundance. Furthermore, protein and transcript abundance were poorly correlated, and the correlation coefficients decreased during fruit development and ripening. Our comprehensive transcriptomic and proteomic data offer a valuable resource for watermelon research, and provide new insights into the molecular mechanisms underlying complex regulatory networks of fruit ripening in watermelon.

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Next Generation Sequencing Facilitates Quantitative Analysis of watermelon fruit small RNA expression

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Evaluation of genes involved in watermelon fruit ripening and development

2008-08-01 | GSE11246 | GEO

Evaluation of genes involved in watermelon fruit ripening and development

Project description:A microarray and quantitative Real-Time PCR-based study was conducted in watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai var. lanatus] in order to elucidate the flow of events associated with fruit development and ripening in this species. RNA from three different maturation stages of watermelon fruit, as well as leaf, were collected from field grown plants during three consecutive years, and hybridized to high-density, photolithography microarrays. Keywords: developmental time course, gene expression This experiment contained a single biological replicate, two tissue types (leaf, fruit flesh), and three time points (12 days post-pollination, 24 days post-pollination, and 36 days post-pollination. One hundred and twenty-seven genes were chosen from this experiments and used in conjunction with quantitative-PCR to examine two additional biological replications of the experiment.

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