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Programmatic Introduction of Parenchymal Cell Types in Blood Vessel Organoids

ABSTRACT: We developed a bottom-up engineering approach to building vascularized human tissue by combining genetic reprogramming with chemically directed organoid differentiation. As a proof of concept, we created neuro-vascular and myo-vascular organoids via transcription factor overexpression in vascular organoids. We characterized neuro-vascular organoids (iN-VOs) by single cell RNA-sequencing (scRNA-seq) to determine cell type composition and gene expression. Using droplet based scRNA-seq we analyzed over 26,000 cells across day 45 organoids grown under three conditions: 45 days of NEUROD1 overexpression (iN-VO, 45 Days Induction), 15 days of NEUROD1 overexpression (iN-VO, 15 Days Induction) and without overexpression (iN-VO, No Induction). We find that organoids contain a neural cell population only upon induction of NEUROD1 while maintaining the full set of endothelial and mesenchymal lineages present in uninduced vascular organoids.

ORGANISM(S): Homo sapiens

PROVIDER: GSE164268 | GEO | 2021/08/20

REPOSITORIES: GEO

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