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Identification of early flg22 responsive genes in Arabidopsis mesophyll cells

ABSTRACT: The goal of this experiment was to identify the early responsive genes activated by the 22 amino acid peptide of bacterial flagellin (flg22) in Arabidopsis mesophyll cells that are involved in the initial responses important for plant innate immunity. We used the microarrays to detail the global program of gene expression underlying intial cellular and molecular responses to microbial associated molecualr patterns (MAMPs).

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE16472 | GEO | 2010/03/18

SECONDARY ACCESSION(S): PRJNA122887

REPOSITORIES: GEO

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Identification of early flg22 responsive genes in Arabidopsis mesophyll cells

Project description:The goal of this experiment was to identify the early responsive genes activated by the 22 amino acid peptide of bacterial flagellin (flg22) in Arabidopsis mesophyll cells that are involved in the initial responses important for plant innate immunity. We used the microarrays to detail the global program of gene expression underlying intial cellular and molecular responses to microbial associated molecualr patterns (MAMPs). Arabidopsis mesophyll protoplasts were treated with 100 nM flg22 for 30 min and 60 min. Transcript profiles were analyzed and compared between control and treated cells to identify early flg22 responsive genes.

2010-03-18 | E-GEOD-16472 | biostudies-arrayexpress

Identification of early flg22 responsive genes in Arabidopsis seedlings

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Identification of early flg22 responsive genes in Arabidopsis seedlings

Project description:The goal of this experiment was to identify the early responsive genes activated by the 22 amino acid peptide of bacterial flagellin (flg22) in Arabidopsis seedlings that are involved in the initial responses important for plant innate immunity. We used the microarrays to detail the global program of gene expression underlying intial cellular and molecular responses to microbial associated molecualr patterns (MAMPs). Arabidopsis seedlings were treated with 2 nM flg22 for 30 min and 60 min. Transcript profiles were analyzed and compared between control and treated cells to identify early flg22 responsive genes.

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Identification of CPK5ac and CPK11ac early target genes in Arabidopsis mesophyll cells

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Identification of CPK5ac and CPK11ac early target genes in Arabidopsis mesophyll cells

Project description:The goal of this experiment was to identify the early target genes of constitutively activated CPK5ac and CPK11ac in Arabidopsis mesophyll cells that are involved in early flagellin responses important for plant innate immunity. We used the microarrays to detail the global program of gene expression underlying initial calcium responses to microbial associated molecualr patterns (MAMPs). Arabidopsis mesophyll protoplasts were transfected with plasmid DNA to express constitutively active CPK5ac or CPK11ac for 6 hr. Transcript profiles were analyzed and compared between cells transfected with control DNA and CPK5ac or CPK11ac DNA to identify early CPK5ac and CPK11ac target genes.

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mpk6 flg22 - Flagellin response in mpk mutants

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Project description:au-09-01_mpk_flagellin_edu - bak1 bkk1 flg22 - Investigate flagellin mpk dependent genes. - 2 weeks Bak1bkk1 plants treated with flg22 1h 1 microM

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Project description:au-09-01_mpk_flagellin_edu - mpk3 flg22 - Investigate flagellin mpk dependent genes - 2 weeks mpk3 plants treated 1 h with 1 microM flg22. Keywords: treated vs untreated comparison

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