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Krüppel-Like Factor 5 Regulates Airway Epithelial Cell Identity and Function.

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE164853 | GEO | 2021/10/07

REPOSITORIES: GEO

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Krüppel-Like Factor 5 Regulates Airway Epithelial Cell Identity and Function [CHIP-seq]

Project description:A complex network of transcription factors regulates genes involved in establishing and maintaining key biological properties of the human airway epithelium. Here we characterize the role of Krüppel-Like Factor 5 (KLF5), in controlling essential pathways of epithelial cell identity and function in the human lung. RNA-seq following siRNA-mediated depletion of KLF5 in the Calu-3 lung epithelial cell line identified significant enrichment of genes encoding chemokines and cytokines involved in the proinflammatory response, and also components of the junctional complexes mediating cell adhesion. To determine direct gene targets of KLF5, we defined the cistrome of KLF5 using ChIP-seq in both Calu-3 and 16HBE14o- lung epithelial cells lines. Occupancy site concordance analysis revealed that KLF5 co-localized with the active histone modification H3K27ac and also with binding sites for CCAAT Enhancer Binding Protein Beta (C/EBPβ). Depletion of KLF5 increased both the expression and secretion of important chemokines, a response that was enhanced following exposure to Pseudomonas aeruginosa lipopolysaccharide. Wound scratch assays in Calu-3 cells exhibited faster rates of repair after KLF5 depletion than did negative controls. Similarly, CRISPR-mediated KLF5-null 16HBE14o- cells also showed enhanced wound closure. These data reveal a pivotal role for KLF5 in coordinating epithelial functions relevant to human lung disease.

2021-10-07 | GSE164852 | GEO

Krüppel-Like Factor 5 Regulates Airway Epithelial Cell Identity and Function [RNA-seq]

2021-10-07 | GSE164851 | GEO

Krüppel-Like Factor 5 Regulates Airway Epithelial Cell Identity and Function.

Project description:Krüppel-Like Factor 5 Regulates Airway Epithelial Cell Identity and Function.

| PRJNA692202 | ENA

Ets homologous factor regulates pathways controlling response to injury in Calu-3 airway epithelial cells

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-12-23 | E-GEOD-63400 | biostudies-arrayexpress

Krüppel-like factor 4 regulates the cytolytic effector function of exhausted CD8 T-cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-10-24 | GSE212806 | GEO

Krüppel-like factor 5 regulates stemness, lineage specification, and regeneration of intestinal epithelial stem cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-10-28 | GSE131278 | GEO

Krüppel-Like Factor 5 Regulates Airway Epithelial Cell Identity and Function [RNA-seq]

Project description:Krüppel-Like Factor 5 Regulates Airway Epithelial Cell Identity and Function [RNA-seq]

| PRJNA692205 | ENA

Krüppel-Like Factor 5 Regulates Airway Epithelial Cell Identity and Function [CHIP-seq]

Project description:Krüppel-Like Factor 5 Regulates Airway Epithelial Cell Identity and Function [CHIP-seq]

| PRJNA692206 | ENA

Ets homologous factor regulates pathways controlling response to injury in Calu-3 airway epithelial cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2014-12-23 | GSE63400 | GEO

Krüppel-like factor 4 regulates T cell function

Project description:Krüppel-like factor 4 (Klf4), a transcription factor mediating context-dependent activation or repression, plays a key role in maintaining pluripotency of stem cells and in regulating cell differentiation. However, the precise function of Klf4 in T cell development and differentiation is largely unknown. Here we showed that Klf4 was highly expressed in thymocyte subsets and mature T cells, and was rapidly down-regulated in mature T cells after activation. In T cell-specific Klf4 conditional knockout mice, we observed a modest reduction of thymocytes (27%). This was due partly to the loss of Klf4 repression of Cdkn1b expression in thymocytes resulting in decreased proliferation of DN thymocytes. Together, these findings identify Klf4 as a critical regulator in T cell development. Klf4fl/fl mice (Katz et al., 2002) were crossed with CD4-Cre mice (Lee et al., 2001) to generate Klf4fl/fl-CD4-Cre+ (Klf4 conditional knockout in T cells) and Klf4fl/fl-CD4-Cre- (control) mice. Mice used for experiments were between 8 and 10 weeks old. Total RNA was extracted from freshly isolated DP+SP4 thymocytes from 3 KO mice and 3 control mice. RNA samples were labeled and hybridized to Illumina Sentrix MouseRef-8 v2 bead arrays.

2011-06-24 | E-GEOD-24880 | biostudies-arrayexpress

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