Single cell RNA sequencing of mock and RV1A infected human airway epithelial organoids
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ABSTRACT: We used single cell RNA sequencing to retrieve transcriptomics of different cell types in human airway epithelial organoids following RV1A infection. By aligning the raw reads to the viral genome, we also quantified viral RNA copies in each cell. The sequencing obtained transcription profile of 6260 cells with 53153 mean reads per cell in the mock sample, and 5727 cells with 61652 mean reads in the RV1A infected sample, before filtering in downstream analysis. We found that RV1A copies is only detected in a small proportion of the entire cell population, however, nearly all cells regardless of cell types showed ISG induction when comparing the mock and RV1A infected sample. We also characterized the expression profile genes related to viral entry: RV1A receptors of LDLR family are ubiquitously expressed in the culture.
ORGANISM(S): Homo sapiens
PROVIDER: GSE164982 | GEO | 2021/05/25
REPOSITORIES: GEO
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