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Mapping genomic loci bound to BRD2 in Calu-3 cells

ABSTRACT: we performed CUT&RUN to comprehensively map genomic loci bound to BRD2 in Calu-3 cells. We also included H3K4me3 and IgG antibodies as postive and negative controls, repectively

ORGANISM(S): Homo sapiens

PROVIDER: GSE165012 | GEO | 2021/01/19

REPOSITORIES: GEO

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BRD2 CUT & RUN in Calu-3 cells

Project description:we performed CUT&RUN to comprehensively map genomic loci bound to BRD2 in control and BRD2 knockdown Calu-3 cells. We also included H2A.Z, H3K4me3 and IgG antibodies.

2021-09-02 | GSE182993 | GEO

BRD2 CUT & RUN in Calu-3 cells

Project description:BRD2 CUT & RUN in Calu-3 cells

| PRJNA758691 | ENA

Mapping genomic loci bound to BRD2 in Calu-3 cells

Project description:Mapping genomic loci bound to BRD2 in Calu-3 cells

| PRJNA692840 | ENA

BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2

Project description:We performed RNA sequencing of Calu-3 cells after treatment with the BET-domain inhibitors JQ-1 and ABBV-744 as well as BRD2 CRISPRi knockdown. We also included CRISPRi knockdown of two other validated hit genes from our screen, COMP and ACE2.

2021-01-19 | GSE165025 | GEO

Brd2 regulates gene expression and alternative splicing.

Project description:We analyzed a role of Brd2 protein in transcription and alternative splicing. 289 genes change alternative splicing after Brd2 knockdown and 1459 genes alter gene expression compared to cells treated with negative control siRNA. 6 samples (3 independent samples of cells treated with Brd2 siRNA, 3 samples of cells treated with negative control siRNA)

2013-12-03 | E-GEOD-39937 | biostudies-arrayexpress

Transcription profiling of mouse BRD2-mediated lymphomas

Project description:The dual bromodomain protein Brd2 is closely related to the basal transcription factor TAFII250, which is essential for cyclin A transactivation and mammalian cell cycle progression. In transgenic mice, constitutive lymphoid expression of Brd2 causes a malignancy most similar to human diffuse large B cell lymphoma. We compare the genome-wide transcriptional expression profiles of these lymphomas with those of proliferating and resting normal B cells. Transgenic tumors reproducibly show differential expression of a large number of genes important for cell cycle control and lymphocyte biology; expression patterns are either tumor-specific or proliferation-specific. Several of their human orthologs have been implicated in human lymphomagenesis. Others correlate with human disease survival time. BRD2 is underexpressed in some subtypes of human lymphoma and these subtypes display a number of similarities to the BRD2-mediated murine tumors. We illustrate with a high degree of detail that cancer is more than rampant cellular proliferation, but involves the additional transcriptional mobilization of many genes, some of them poorly characterized, which show a tumor-specific pattern of gene expression. Experiment Overall Design: 26 samples are included in this series. Sporadic murine E-mu-BRD2 mediated lymphomas are divided into three classes by disease severity and compared to either resting or mitogen-activated B Cells. The resting and activated B Cells are either wildtype or E-mu-BRD2 transgenic. All samples are on the FVB wildtype background.

2008-06-14 | E-GEOD-6136 | biostudies-arrayexpress

Prochlorothrix hollandica PCC 9006 = CALU 1027 isolate:CALU 1027

Project description:Prochlorothrix hollandica PCC 9006 = CALU 1027 isolate:CALU 1027 Genome sequencing and assembly

| PRJNA63021 | ENA

Brd2 regulates gene expression and alternative splicing.

2013-12-03 | GSE39937 | GEO

BRD2 interconnects with BRD3 to facilitate Pol II transcription initiation and elongation to prime promoters for cell differentiation

Project description:Understanding the precise functions and relationship of BRD2 with other bromodomain and extraterminal motif (BET) proteins is central for the application of BET-specific and pan inhibitors. Here, we used acute protein degradation and quantitative genomic and proteomic approaches to investigate the primary functions of BRD2 in transcription. We report that BRD2 is required for TAF3-mediated Pol II initiation at low levels of H3K4me3-modified promoters and Pol II elongation by suppressing R-loops. Single and double depletion revealed that BRD2 and BRD3, but not BRD4, redundantly and independently function in Pol II transcription at different promoters and cooperatively occupy enhancers. Interestingly, we found that depletion of BRD2 affects the expression of different genes during differentiation processes, priming with promoter regulation in ES cells. Therefore, our results suggest complex interconnections between BRD2 and BRD3 at promoters to fine-tune Pol II initiation and elongation for control of cell state.

2024-05-22 | PXD030774 | Pride

Haploinsufficiency in bromodomain containing 2 (Brd2) gene remodels synaptic transmission in male mouse striatum

Project description:Our previous work confirmed that Brd2+/- mouse presents the sex specific characteristics of juvenile myoclonic epilepsy, a subsyndrome of idiopathic generalized epilepsy. Here, we investigated the role of Brd2 gene in modulating the genomic fabrics responsible for the glutamatergic, GABAergic, dopaminergic, cholinergic and serotonergic transmission in the front cortex striata of male mice. Agilent two-color gene expression arrays and the genomic fabric paradigm were used to determine whether the expression and networking of genes involved in the synaptic transmission differ between Brd2+/- and wildtype mice. We found relatively few regulated genes in Brd2+/-mice with respect to their wildtype counterparts. However, most of the regulated genes were directly related to control of anxiety and seizures, justifying the role of Brd2 upstream control of these traits. Agilent mouse two-color gene expression arrays to profile striata of the prefrontal cortices of wildtype (W) and Brd2+/- (H) mouse male (M) adult C57Bl/6j mice. Differently labeled biological replicates were cohybridized with each array. Results of similarly labeled different conditions were compared then averaged for the two fluorescent labels. For instance: MW1 & MW3 were compared with MH1 & MH3, MW2 & MW4 were compared with MH2 & + MH4, and the results of the comparisons averaged. This design uses 100% of the resources, has a better normlaiztion and allows all possible comparisons among the conditions.

2015-09-01 | E-GEOD-72562 | biostudies-arrayexpress

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