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MRNA samples in Allele Specific Expression study

ABSTRACT: Col-0 and Van-0 total RNA were mixed at 1:1. Total RNA also extracted from F1 hybrid samples from Col x Van and Van x Col. PolyA RNA were purified and double-strand cDNA were synthesized, followed by bioprime random labeling. A total of 16ug labelled products were hybridized to AtSNPtile1. cis regulatory effect was identified as allele specific expression in F1 hybrids. Composite trans regulatory effect was detected as deviation between parental expression and F1 hybrids expression.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE16506 | GEO | 2009/08/15

SECONDARY ACCESSION(S): PRJNA122911

REPOSITORIES: GEO

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mRNA samples in Allele Specific Expression study

Project description:Col-0 and Van-0 total RNA were mixed at 1:1. Total RNA also extracted from F1 hybrid samples from Col x Van and Van x Col. PolyA RNA were purified and double-strand cDNA were synthesized, followed by bioprime random labeling. A total of 16ug labelled products were hybridized to AtSNPtile1. cis regulatory effect was identified as allele specific expression in F1 hybrids. Composite trans regulatory effect was detected as deviation between parental expression and F1 hybrids expression. Each sample type has four replicates. Single chanel. Three sample types (Col and Van RNA 1:1 mixture, Col x Van F1 hybrids, Van x Col F1 hybrids). Total 12 samples.

2010-05-06 | E-GEOD-16506 | biostudies-arrayexpress

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Gene expression of Col, Van and reciprocal hybrids

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Project description:Genomic DNA of Col-0 and Van-0 were mixed at 1:1 and labeled using Bioprime randon labeling kit. A total of 16ug labelled products were hybridized to AtSNPtile1. Used as the reference of equal allele expression. Col and Van genomic DNA were mixed at 1:1 with four biological replicates. Single chanel. Total 4 samples.

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