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Heat shock-mediated global gene downregulation is achieved by premature transcription termination

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE165368 | GEO | 2022/01/24

REPOSITORIES: GEO

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Heat shock-mediated global gene downregulation is achieved by premature transcription termination [4SU_mRNA-seq]

Project description:The Heat Shock response (HSR) is a highly conserved transcriptional program induced by the exposure to a variety of environmental stressors. Following an insult, a small subset of genes, known as the Heat Shock genes, are rapidly induced by the Heat Shock Factors (HSFs) to maintain protein homeostasis and ensure cell survival. In parallel, a large portion of the genome becomes repressed. HS associated global gene repression is still largely poorly understood. In this study, we demonstrate that transcription downregulation during Heat shock is mainly achieved by premature transcription termination through the regulation of U1snRNA and the disruption of U1-telescripting.

2022-01-24 | GSE165367 | GEO

Heat shock-mediated global gene downregulation is achieved by premature transcription termination [FP_TTchem-seq]

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Heat shock-mediated global gene downregulation is achieved by premature transcription termination [DRB_TTchem-seq]

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Heat shock-mediated global gene downregulation is achieved by premature transcription termination [TTchem-seq]

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Heat shock-mediated global gene downregulation is achieved by premature transcription termination

Project description:Heat shock-mediated global gene downregulation is achieved by premature transcription termination

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Heat shock-mediated global gene downregulation is achieved by premature transcription termination [FP_TTchem-seq]

Project description:Heat shock-mediated global gene downregulation is achieved by premature transcription termination [FP_TTchem-seq]

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Heat shock-mediated global gene downregulation is achieved by premature transcription termination [DRB_TTchem-seq]

Project description:Heat shock-mediated global gene downregulation is achieved by premature transcription termination [DRB_TTchem-seq]

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Heat shock-mediated global gene downregulation is achieved by premature transcription termination [TTchem-seq]

Project description:Heat shock-mediated global gene downregulation is achieved by premature transcription termination [TTchem-seq]

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Heat shock-mediated global gene downregulation is achieved by premature transcription termination [4SU_mRNA-seq]

Project description:Heat shock-mediated global gene downregulation is achieved by premature transcription termination [4SU_mRNA-seq]

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Antisense-Mediated Transcript Knockdown Triggers Premature Transcription Termination.

Project description:Antisense oligonucleotides (ASOs) that trigger RNase-H-mediated cleavage are commonly used to knock down transcripts for experimental or therapeutic purposes. In particular, ASOs are frequently used to functionally interrogate long noncoding RNAs (lncRNAs) and discriminate lncRNA loci that produce functional RNAs from those whose activity is attributable to the act of transcription. Transcription termination is triggered by cleavage of nascent transcripts, generally during polyadenylation, resulting in degradation of the residual RNA polymerase II (Pol II)-associated RNA by XRN2 and dissociation of elongating Pol II. Here, we show that ASOs act upon nascent transcripts and, consequently, induce premature transcription termination downstream of the cleavage site in an XRN2-dependent manner. Targeting the transcript 3' end with ASOs, however, allows transcript knockdown while preserving Pol II association with the gene body. These results demonstrate that the effects of ASOs on transcription must be considered for appropriate experimental and therapeutic use of these reagents.

| S-EPMC7093083 | biostudies-literature

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