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Involvement of UBA2C in ambient temperature-dependent flowering by promoting FLM transcription [ChIP-seq]

ABSTRACT: Ambient temperature dependent flowering time is an important feature that is required for plants to reproduce in various environmental conditions. The rising global temperature poses a significant challenge to the growth and reproduction of plants. In Arabidopsis thaliana, FLM-β, a major splicing isoform of the flowering repressor gene FLOWERING LOCUS M, is down-regulated in response to increasing temperature and represents a critical mechanism for plants to respond to temperature changes. However, it is unknown how the transcript level of FLM-β is down-regulated. Here we identify an RRM domain-containing protein, UBA2C, as a previously uncharacterized flowering repressor by forward genetic screening. We demonstrate that UBA2C directly binds to FLM chromatin and facilitates FLM transcription predominantly by inhibiting the histone H3K27 trimethylation, a histone mark related to transcriptional repression. At FLM chromatin, the histone H3K27 trimethylation is enhanced in response to increasing temperatures. Depletion of UBA2C weakens the response of FLM transcription and H3K27 trimethylation to temperature changes. UBA2C forms multiple puncta in the nucleus and the number of puncta increases with increasing temperatures. UBA2C contains a prion-like domain (PrLD) that is responsible for forming puncta in the nucleus in vivo. These results not only identify a previously unknown flowering-time regulator but also reveal the mechanism by which the regulator controls flowering time in response to temperature changes.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE165432 | GEO | 2021/10/28

REPOSITORIES: GEO

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Involvement of UBA2C in ambient temperature-dependent flowering by promoting FLM transcription [RNA-seq]

Project description:Ambient temperature dependent flowering time is an important feature that is required for plants to reproduce in various environmental conditions. The rising global temperature poses a significant challenge to the growth and reproduction of plants. In Arabidopsis thaliana, FLM-β, a major splicing isoform of the flowering repressor gene FLOWERING LOCUS M, is down-regulated in response to increasing temperature and represents a critical mechanism for plants to respond to temperature changes. However, it is unknown how the transcript level of FLM-β is down-regulated. Here we identify an RRM domain-containing protein, UBA2C, as a previously uncharacterized flowering repressor by forward genetic screening. We demonstrate that UBA2C directly binds to FLM chromatin and facilitates FLM transcription predominantly by inhibiting the histone H3K27 trimethylation, a histone mark related to transcriptional repression. At FLM chromatin, the histone H3K27 trimethylation is enhanced in response to increasing temperatures. Depletion of UBA2C weakens the response of FLM transcription and H3K27 trimethylation to temperature changes. UBA2C forms multiple puncta in the nucleus and the number of puncta increases with increasing temperatures. UBA2C contains a prion-like domain (PrLD) that is responsible for forming puncta in the nucleus in vivo. These results not only identify a previously unknown flowering-time regulator but also reveal the mechanism by which the regulator controls flowering time in response to temperature changes.

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