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Next Generation Sequencing Facilitates Quantitative Analysis of B cell Transcriptomes

ABSTRACT: Emergence of IgE antibodies that bind allergens with high affinity is highly correlated with the severity of anaphylaxis. However, the underlying molecular mechanisms by which high-affinity IgE is generated remain poorly understood. To examine function of IL-13 derived from T cells in IgE producing B cell development, we transferred IL-13 sufficient or deficient OT-2 TH2 into IgE reporter (Verigem) mice followed by immunization with NP-OVA. At 7 day post immunization, NP specific IgE producing B cells were sorted and served to single cell transcriptome analysis.

ORGANISM(S): Mus musculus

PROVIDER: GSE165539 | GEO | 2021/01/27

REPOSITORIES: GEO

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