Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression analyses of mir-30a knockdown in ovarian clear cell cancer cell line

ABSTRACT: A variety of human cancers demonstrate alterations in microRNA expression. We hypothesized that regulatory defects in microRNAs play a central early role in organizing the molecular changes involved in ovarian cancer (OvCa). Using both gene arrays and deep sequencing, we comprehensively profiled mRNA and microRNA expression, respectively, in human clear cell epithelial OvCa cell lines and short-term primary cultures of normal ovarian surface epithelium. We expected that over-expression of a specific microRNA would lead to lower expression of its mRNA targets, and under-expression of a specific microRNA would lead to higher expression of its target genes. Using our expression data in conjunction with established in silico algorithms, we found putative microRNA:mRNA functional pairs. Furthermore, gene expression profiles were taken of clear cell cultures having functional knockdown or over-expression of specific microRNAs of interest. Knockdown of mir-30a (found over-expressed in clear cell OvCa) resulted in up-regulation in vitro of a significant number of the in silico predicted mir-30a target genes that were normally under-expressed in OvCa. Keywords: two group comparison; gene expression profiling

ORGANISM(S): Homo sapiens

PROVIDER: GSE16569 | GEO | 2010/01/28

SECONDARY ACCESSION(S): PRJNA122785

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene expression analyses of mir-22 overexpression in ovarian clear cell cancer cell line

Project description:A variety of human cancers demonstrate alterations in microRNA expression. We hypothesized that regulatory defects in microRNAs play a central early role in organizing the molecular changes involved in ovarian cancer (OvCa). Using both gene arrays and deep sequencing, we comprehensively profiled mRNA and microRNA expression, respectively, in human clear cell epithelial OvCa cell lines and short-term primary cultures of normal ovarian surface epithelium. We expected that over-expression of a specific microRNA would lead to lower expression of its mRNA targets, and under-expression of a specific microRNA would lead to higher expression of its target genes. Using our expression data in conjunction with established in silico algorithms, we found putative microRNA:mRNA functional pairs. Furthermore, gene expression profiles were taken of clear cell cultures having functional knockdown or over-expression of specific microRNAs of interest. Knockdown of mir-22 (found under-expressed in clear cell OvCa) resulted in down-regulation in vitro of a significant number of the in silico predicted mir-22 target genes that were normally over-expressed in OvCa. Keywords: two group comparison; gene expression profiling

2010-01-28 | GSE16568 | GEO

Gene expression analyses of mir-182 knockdown in ovarian clear cell cancer cell line

Project description:A variety of human cancers demonstrate alterations in microRNA expression. We hypothesized that regulatory defects in microRNAs play a central early role in organizing the molecular changes involved in ovarian cancer (OvCa). Using both gene arrays and deep sequencing, we comprehensively profiled mRNA and microRNA expression, respectively, in human clear cell epithelial OvCa cell lines and short-term primary cultures of normal ovarian surface epithelium. We expected that over-expression of a specific microRNA would lead to lower expression of its mRNA targets, and under-expression of a specific microRNA would lead to higher expression of its target genes. Using our expression data in conjunction with established in silico algorithms, we found putative microRNA:mRNA functional pairs. Furthermore, gene expression profiles were taken of clear cell cultures having functional knockdown or over-expression of specific microRNAs of interest. Knockdown of mir-182 (found over-expressed in clear cell OvCa) resulted in up-regulation in vitro of a significant number of the in silico predicted mir-182 target genes that were normally under-expressed in OvCa. Keywords: two group comparison; gene expression profiling

2010-01-28 | GSE16572 | GEO

Gene expression analyses of mir-100 overexpression in ovarian clear cell cancer cell line

Project description:A variety of human cancers demonstrate alterations in microRNA expression. We hypothesized that regulatory defects in microRNAs play a central early role in organizing the molecular changes involved in ovarian cancer (OvCa). Using both gene arrays and deep sequencing, we comprehensively profiled mRNA and microRNA expression, respectively, in human clear cell epithelial OvCa cell lines and short-term primary cultures of normal ovarian surface epithelium. We expected that over-expression of a specific microRNA would lead to lower expression of its mRNA targets, and under-expression of a specific microRNA would lead to higher expression of its target genes. Using our expression data in conjunction with established in silico algorithms, we found putative microRNA:mRNA functional pairs. Furthermore, gene expression profiles were taken of clear cell cultures having functional knockdown or over-expression of specific microRNAs of interest. Knockdown of mir-100 (found under-expressed in clear cell OvCa) resulted in down-regulation in vitro of a significant number of the in silico predicted mir-100 target genes that were normally over-expressed in OvCa. Keywords: two group comparison; gene expression profiling

2010-01-28 | GSE16571 | GEO

Gene expression analyses of ovarian clear cell cancer cell lines

Project description:A variety of human cancers demonstrate alterations in microRNA expression. We hypothesized that regulatory defects in microRNAs play a central early role in organizing the molecular changes involved in ovarian cancer (OvCa). Using both gene arrays and deep sequencing, we comprehensively profiled mRNA and microRNA expression, respectively, in human clear cell epithelial OvCa cell lines and short-term primary cultures of normal ovarian surface epithelium. We expected that over-expression of a specific microRNA would lead to lower expression of its mRNA targets, and under-expression of a specific microRNA would lead to higher expression of its target genes. Using our expression data in conjunction with established in silico algorithms, we found putative microRNA:mRNA functional pairs. Keywords: two group comparison; gene expression profiling

2010-01-28 | GSE16570 | GEO

Gene expression analyses of mir-31 overexpression in ovarian serous cancer cell line

Project description:A variety of human cancers demonstrate alterations in microRNA expression. We hypothesized that regulatory defects in microRNAs play a central early role in organizing the molecular changes involved in ovarian cancer (OvCa). Using both gene arrays and deep sequencing, we comprehensively profiled mRNA and microRNA expression, respectively, in human serous epithelial OvCa cell lines, serous tumors, and short-term primary cultures of normal ovarian surface epithelium (NOSE). We expected that over-expression of a specific microRNA would lead to lower expression of its mRNA targets, and under-expression of a specific microRNA would lead to higher expression of its target genes. Using our expression data in conjunction with established in silico algorithms, we found putative microRNA:mRNA functional pairs. Furthermore, gene expression profiles were taken of serous cultures having functional knockdown or over-expression of specific microRNAs of interest. Over-expression of mir-31 (found under-expressed in serous OvCa) resulted in down-regulation in vitro of a significant number of the in silico predicted mir-31 target genes. Keywords: two group comparison

2010-02-25 | GSE16700 | GEO

Gene expression analysis of ovarian serous adenocarcinoma cell lines and tumors

2010-02-25 | GSE16708 | GEO

Analysis of gene expression in lung adenocarcinoma cells following over-expression of miR-30a-5p or miR-30a-3p

Project description:RNA-sequencing was performed to gain insight into the transcriptome-wide molecular changes induced by miR-30a-5p or miR-30a-3p over-expression in lung adenocarcinoma cells. Following transfection of miR-30a-5p or miR-30a-3p miRNA mimic or control RNA, RNA-sequencing was performed. This high-throughput data revealed elevated expression of both miR-30a-5p and miR-30a-3p reduced the expression of genes and pathways known to induce proliferation and movement in lung adenocarcinoma cells.

2020-02-26 | GSE142695 | GEO

Analysis of gene expression in lung adenocarcinoma cells following over-expression of miR-30a-5p or miR-30a-3p

Project description:Analysis of gene expression in lung adenocarcinoma cells following over-expression of miR-30a-5p or miR-30a-3p

| PRJNA597999 | ENA

Difference expression analyses before and after human fucosyltransferase gene 1 transfection into ovarian cancer clear cell line

Project description:75% ovarian epithelial tumor overexpresses Lewis y antigen, which notably strenthen such malignant biological behaviors as metastasis, drug resistance and proliferation. The key enzyme of Lewis y synthesis is human fucosyltransferase gene 1(hfut1). Through transfecting hfut1 into Lewis y low expressing cell line RMG-I, we got a pair of cell lines. We hypothesized that Lewis y antigen plays a major role in ovarian canceration . Using gene arrays, we comprehensively profiled mRNA differential expression between fut1-pretransfected and posttransfected clear cell of human epithelial OvCa. We expected that over-expression of lewis y antigen would lead to higher expression of genes associated with growth, metastasis,proliferation and so on.

2012-08-02 | GSE34396 | GEO

Difference expression analyses before and after human fucosyltransferase gene 1 transfection into ovarian cancer clear cell line (Human cancer array)

2012-08-02 | GSE39216 | GEO