Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from normal adults and fetal human thyroids

ABSTRACT: Transcriptomic signature of human embryonic thyroid is not described so far. The goal of this project was to evaluate what enables the transition from differentiation to functional maturation of the human developing thyroid, by performing transcriptomic analysis of human thyroids covering the period of gestation weeks 7-11 and comparing it to adult human thyroids. We defined a non TSH (thyroid stimulating hormone) dependent transition from differentiation to maturation of thyroid. The study also sought to shed light on possible factors that could replace TSH, which is absent in this window of gestational age, to trigger transition to the emergence of thyroid function.

ORGANISM(S): Homo sapiens

PROVIDER: GSE165706 | GEO | 2021/07/15

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

miRNA and gene expression profiling in human thyroid carcinomas and non-neoplastic thyroids [RNA]

Project description:We performed miRNA and gene expression profiling in a series of 30 thyroid carcinomas and 6 non-neoplastic thyroids.

2020-09-30 | GSE104005 | GEO

miRNA and gene expression profiling in human thyroid carcinomas and non-neoplastic thyroids [miRNA]

Project description:We performed miRNA and gene expression profiling in a series of 30 thyroid carcinomas and 6 non-neoplastic thyroids.

2020-09-30 | GSE103996 | GEO

Transcriptional response of mouse thyroids following in vivo 211At exposure reveals distinct gene expression profiles

Project description:Transcriptomic profiling of normal mouse thyroid tissue following 211At irradiation Astatine-211 (211At) is an alpha particle emitting halogen with almost optimal linear energy transfer (LET) for creating DNA double strand breaks, and is thus proposed for radionuclide therapy when bound to tumor-seeking agents. Un-bound 211At accumulates in the thyroid gland. The concept of basal radiation-induced biological effects in thyroid tissue is to a high degree unknown and is most valuable. Female BALB/c nude mice were i.v. injected with 0.064-42 kBq of 211At resulting in absorbed doses to the thyroid gland of 0.05-32 Gy. Thyroids were removed at 24 h after injection and total RNA was extracted from pooled thyroids and processed in triplicate using Illumina MouseRef-8 Whole-Genome Expression Beadchips. Nexus Expression 2.0 was used for data analysis. Thyroids exposed to 211At revealed distinctive gene expression profiles compared to non-irradiated controls. More genes were affected at low absorbed doses (0.05 and 0.5 Gy) compared to intermediate (1.4 Gy) and high absorbed doses (11 and 32 Gy) and the proportion of dose-specific genes increased with decreased absorbed dose. This result might be the manifestation of increased heterogeneous irradiation with decreasing absorbed dose, indicating a bystander effect. Also, 1.4 Gy often had an opposite regulation compared to the other absorbed doses. No inflammatory effects were seen while 0.05 and 11 Gy affected the immune system. Effects on the cellular response to outer stress and cell cycle regulation and proliferation were seen at 1.4 and 11 Gy. These results indicate that the cellular response to ionizing radiation is complex and differs with absorbed doses. Total RNA was isolated from fresh-frozen tissue samples (Normal balb/c mouse thyroids)

2013-02-13 | E-GEOD-32306 | biostudies-arrayexpress

Expression data from normal adults and fetal human thyroids

Project description:Expression data from normal adults and fetal human thyroids

| PRJNA701663 | ENA

Transcriptomic analysis reveals a critical role for activating Gsα mutations in spontaneous feline hyperthyroidism

Project description:Feline hyperthyroidism (FHT) is a debilitating disease affecting >10% of elderly cats. It is generally characterised by chronic elevation of thyroid hormone in the absence of circulating TSH. Understanding of the molecular pathogenesis of FHT is currently limited. However, FHT shares clinical and histopathological similarities with human toxic multinodular goitre, which has been associated with activating mutations in TSH receptor (TSHR) and Gsα encoding genes. Using RNA-seq transcriptomic analysis of thyroid tissue from hyperthyroid and euthyroid cats, we identified differentially expressed genes and dysregulated pathways in FHT, many of which are downstream of TSHR. In addition, we detected missense variants in thyroid RNA-seq reads that alter the structure of both TSHR and Gsα. All FHT-associated mutations were absent in germline sequence from paired blood samples. Only a small number of hyperthyroid cats demonstrated TSHR variation, however all thyroids from advanced cases of FHT carried at least one missense variant affecting Gsα. The activating nature of the acquired Gsα mutations was demonstrated by increased cAMP production in vitro. These data indicate that constitutive activation of signalling downstream of TSHR is central to the TSH-independent production of thyroid hormone in FHT, offering a novel therapeutic target pathway in this common disease.

2024-09-07 | GSE276271 | GEO

Somatic CNV profile of congenital ectopic thyroids

Project description:To assess whether specific genes have relevant somatic genetic or epigenetic alterations in ectopic tissue, we used a combined analysis of transcriptome (confirmed by qRT-PCR on 68 genes), methylome, structural genome variations (copy number variants, CNVs) and miRNA profile of ectopic compared with orthotopic thyroids. DNA form three ectopic thyroid compared with matched leucocytes.

2010-10-27 | E-GEOD-17463 | biostudies-arrayexpress

Transcriptional response of mouse thyroids following in vivo 211At exposure reveals distinct gene expression profiles

2013-02-13 | GSE32306 | GEO

pool of thyroids from wild type, E7 and RET-PTC tg mice at 2, 6 and 10 months

Project description:The RET/PTC3 (RP3) fusion gene is the most frequent mutation found in radiation-induced papillary thyroid cancers (PTC). Several studies suggest that the RET/PTC rearrangement is an initiating event in tumorigenesis. E7 is an oncoprotein derived from the Human Papilllomavirus 16 (HPV16) responsible for most cervical carcinoma in women. We studied here the sequence of events leading to thyroid cancer in Tg-RP3 and Tg-E7 mice expressing the transgene exclusively in the thyroid under the control of thyroglobulin (Tg) promoter. Both transgenic mice develop thyroid hyperplasia followed by solid differentiated carcinoma in older animals. To understand the different steps leading to carcinoma, we analyzed thyroid gene expression in both strains at different ages (2, 6, 10 months) by microarray technology. Important biological processes were differentially regulated in the two tumor types. In E7 thyroids cell cycle was the most upregulated process; observation consistent with the huge size of these tumors. In RP3 thyroids immunity was the most significantly regulated process, as previously observed in microarray data on human PTC. Interestingly, other human PTC characteristics were also observed in RP3 but not in E7 mouse tumors: similar regulation of several human PTC markers, upregulation of many EGF-like growth factors and finally significant regulation of angiogenesis and extracellular matrix remodeling-related genes. In summary we showed that RP3 contrary to E7 mouse tumors share several important genotypic characteristics with human PTC, observation reinforcing the validity of this model to study human thyroid tumorigenesis. Keywords: time course, genetic modification, disease state, cancer

2008-07-18 | GSE10743 | GEO

miRNA and gene expression profiling in human thyroid carcinomas and non-neoplastic thyroids

Project description:miRNA and gene expression profiling in human thyroid carcinomas and non-neoplastic thyroids

| PRJNA407930 | ENA

miRNA and gene expression profiling in human thyroid carcinomas and non-neoplastic thyroids [RNA]

Project description:miRNA and gene expression profiling in human thyroid carcinomas and non-neoplastic thyroids [RNA]

| PRJNA407936 | ENA