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Systems-wide analysis of the GATC-binding nucleoid-associated protein Gbn and its impact on Streptomyces development

ABSTRACT: We report the DNA binding profile of a novel family of nucleoid-associated protein in Streptomyces coelicolor

ORGANISM(S): Streptomyces coelicolor

PROVIDER: GSE165795 | GEO | 2022/04/02

REPOSITORIES: GEO

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Systems-wide analysis of the GATC-binding nucleoid-associated protein Gbn and its impact on Streptomyces development

Project description:We report the regulatory effect of a novel family of nucleoid-associated protein in Streptomyces coelicolor

2022-04-02 | GSE186136 | GEO

Proteomic survey of the Streptomyces coelicolor nucleoid

Project description:Nucleoid-associated proteins (NAPs) are small, highly abundant regulators with low sequence specificity and pleiotropic mutant phenotypes. They are involved in transcriptional and post-transcriptional control of gene expression, DNA protection/repair and nucleoid structuring. Discovery of the major NAPs has been largely haphazard and based with the Enterobacteriaceae, although some Actinomycete-specific NAPs (Mdp1, Lsr2, sIHF) are known. Here, we use LC-MS/MS to systematically search for novel NAPs in isolated nucleoids of the model actinomycete Streptomyces coelicolor. Based on the criteria of high abundance (emPAI) and predicted DNA-binding ability (DNAbinder) we identified a set of around twenty proteins with a good probability of being NAPs. The approach was apparently successful as the set included known major NAPs HupA, sIHF and Lsr2 as well as the global regulators BldD and CRP. It also included a number of proteins whose functions are not yet known (SCO0204, SCO3013, SCO4232, SCO4199 and SCO1839) which provide a useful set of candidates for further study.

2013-04-24 | PRD000736 | Pride

Genome-wide binding site profiling of NdgR in Streptomyces coelicolor

Project description:We identified genome-wide binding regions of NdgR in Streptomyces coelicolor using chromatin immunoprecipitation sequencing (ChIP-seq). We constructed 6×myc-tagged NdgR strain using homologous recombination with myc-tagging vector. Analysis of the sequencing data aligned to Streptomyces coelicolor genome database (NC_003888).

2014-12-29 | GSE59010 | GEO

Systems-wide analysis of the GATC-binding nucleoid-associated protein Gbn and its impact on Streptomyces development

Project description:Systems-wide analysis of the GATC-binding nucleoid-associated protein Gbn and its impact on Streptomyces development

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Systems-wide analysis of the GATC-binding nucleoid-associated protein Gbn and its impact on Streptomyces development

Project description:Systems-wide analysis of the GATC-binding nucleoid-associated protein Gbn and its impact on Streptomyces development

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The regulation and role of SCO2027, iron importer protein in Streptomyces coelicolor

Project description:We determined genes that directly or indirectly regulated by CatR (or PerR), and hydrogen peroxide regulon in Streptomyces coelicolor.

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Differential impact of phosphate availability on primary and specialized metabolism of Streptomyces lividans and Streptomyces coelicolor.

Project description:In order to define the impact of phosphate (Pi) availability on cellular metabolism the project aimed to perform a comparative analysis of the proteomes of two Streptomyces strains with different abilities to produce antibiotics, S. coelicolor and S. lividans as well as of the pptA mutant of S. lividans, grown low (1mM) and high (5mM) phosphate (Pi) availability conditions. Interestingly, in contrast to most Streptomyces species, S. coelicolor produces more antibiotics in Pi proficiency than in Pi limitation, S. lividans does not produce antibiotics in any Pi conditions and the pptA mutant produces antibiotics only in Pi limitation. This in-depth proteomic comparison of three Streptomyces strains (S. coelicolor, S. lividans wt and pptA mutant), in different growth conditions (time and Pi concentration in the medium) was performed on four biological replicates. Protein abundance changes were determined using two label-free mass spectrometry based-quantification methods: spectral count (SC) and MS1 ion intensities named XIC (for eXtracted Ion Current). Our proteomic data reveal for the first time, the impact of Pi availability on the abundance of approximately 4000 proteins of these Streptomyces strains with different abilities to produce antibiotics. The most striking feature differentiating these strains was the much higher abundance of enzymes of the respiratory chain in both phosphate conditions in S. coelicolor compared to the S. lividans strains.

2022-02-04 | PXD029263 | Pride

Effects of cAMP supplementation on the transcriptome of Streptomyces coelicolor M1146 strain

Project description:To identify unique gene expression in cAMP supplemented Streptomyces coelicolor M1146 strain. The genes with different gene expression might be key genes to understand the effects of cAMP supplementation on the transcriptome of Streptomyces coelicolor M1146.

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Effects of cAMP supplementation on the transcriptome of Streptomyces coelicolor M145 strain

Project description:To identify unique gene expression in cAMP supplemented Streptomyces coelicolor M145 strain. The genes with different gene expression might be key genes to understand the effects of cAMP supplementation on the transcriptome of Streptomyces coelicolor M145.

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The genome of Streptomyces rimosus shows a novel structure compared to other Streptomyces

Project description:This study compared the genome of Streptomyces rimosus rimosus against that of Streptomyces coelicolor. It also compared 4 strains with changes in oxytetracycline production and derived from G7, the type strain, against G7. Keywords: Comparative genomic hybridization

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