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Phytogenics from sage and lemon verbena promote innate immunity and barrier function in the gut of gilthead seabream (Sparus aurata)

ABSTRACT: Deciphering the dietary immunomodulatory effects of a medicinal plant leaf extract (MPLE) obateined from sage (Salvia officinalis, Lamiaceae) and lemon verbena (Lippia citriodora, Verbenaceae) upon the gut-associated lymphoid tissue (GALT) of gilthead seabream (Sparus aurata).

ORGANISM(S): Sparus aurata

PROVIDER: GSE166558 | GEO | 2021/06/02

REPOSITORIES: GEO

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Phytogenics from sage and lemon verbena promote innate immunity and barrier function in the gut of gilthead seabream (Sparus aurata)

Project description:Phytogenics from sage and lemon verbena promote innate immunity and barrier function in the gut of gilthead seabream (Sparus aurata)

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Project description:In the present study, the Agilent-016251 Sparus aurata oligo microarray platform (GPL6467) was used to compare expression profiles of mineralization-induced VSa13 cell cultures against untreated ones. ECM mineralization was induced for 4 weeks by supplementing medium with 50 µg/ml of L-ascorbic acid, 10 mM β glycerophosphate and 4 mM CaCl2. For each group, total RNA was extracted from three (3) independent biological replicates, each consisting of pools of cells. Data analysis demonstrated that expression profiles were strongly affected by ECM mineralization with hundreds of genes differentially expressed with relevant fold-change.

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