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Next Generation Sequencing Facilitates Quantitative Analysis of Mock/MASCp36 infected mouse lung Transcriptomes

ABSTRACT: The ongoing SARS-CoV-2 pandemic has brought an urgent need for animal models to study the pathogenicity of the virus. Herein, we generated and characterized a novel mouse-adapted SARS-CoV-2 strain named MASCp36 that causes acute respiratory symptoms and mortality in standard laboratory mice. Particularly, this model exhibits age and gender related skewed distribution of mortality akin to severe COVID-19, and the 50% lethal dose (LD50) of MASCp36 was ~100 PFU in aged, male BALB/c mice. To characterize the transcriptional response to infection of MASCp36 in BALB/c mice (different age and gender), RNA-Seq analysis were performed using lung homogenates collected at 1 and 4 dpi.

ORGANISM(S): Mus musculus

PROVIDER: GSE166778 | GEO | 2021/07/12

REPOSITORIES: GEO

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