Transcriptomics

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In vivo multiplexed gene activation via improved Cas12a


ABSTRACT: To evaluate the specificity of CRISPR activation by vgdCas12a on a genome-wide scale, we carried out whole-transcriptome RNA-seq of HEK293T cells with the TRE3G-GFP reporter transfected with either WT dCas12a or vgdCas12a combined with the TRE3G-targeting crRNA. We also included a non-targeting crRNA as negative control for each case.

ORGANISM(S): Homo sapiens

PROVIDER: GSE166817 | GEO | 2022/01/14

REPOSITORIES: GEO

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