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Single cell trajectory mapping of human pluripotent stem cells differentiating towards lung and hepatocyte progenitors

ABSTRACT: The molecular details for the specification of lung progenitors from human pluripotent stem cells (hPSCs) are unclear. Here, we use single cell RNA-sequencing with high temporal resolution along an optimized differentiation protocol to determine the transcriptional hierarchy of lung specification from human hPSCs. To chart a comprehensive map of the transcriptional states underlying the differentiation of lung progenitors and hepatoblasts in parallel, we performed a 16-day time-series scRNA-seq using the Drop-Seq workflow (Macosko et al., 2015). Single cell suspensions were processed daily, resulting in the analysis of a total of 10,667 cells that were used for downstream analysis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE167011 | GEO | 2023/04/17

REPOSITORIES: GEO

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