Sphingolipids Control Dermal Fibroblast Heterogeneity
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ABSTRACT: Human cells produce thousands of lipids that impact biological processes in ways we are only starting to characterize. The cellular composition in lipids changes during differentiation and also varies across individual cells of the same type. Yet, whether and how cell-to-cell differences in lipid composition affect cell phenotypes remain unknown. Here we have measured the lipidomes and transcriptomes of individual human dermal fibroblasts by coupling high-resolution mass spectrometry imaging to single-cell transcriptomics. We find that the cell-to-cell variation of specific lipid metabolic pathways contributes to the establishment of cell states involved in the organization of skin architecture. In fact, sphingolipid composition defines fibroblast subpopulations and its metabolic rewiring drives cell state transitions. These data uncover a role for cell-to-cell lipid heterogeneity in the determination of cell states and reveal a new regulatory component to the self-organization of multicellular systems.
ORGANISM(S): Homo sapiens
PROVIDER: GSE167209 | GEO | 2021/02/23
REPOSITORIES: GEO
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