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High-throughput Sequencing Facilitates Quantitative Analysis of Transcriptome Difference Underlying Quercetin Antioxidant Action in Ox-LDL-induced HUVECs Damage [miRNA-Seq]

ABSTRACT: High-throughput Sequencing has revolutionized system-based analysis of cellular pathways. The goals of this study are to find the effect of quercetin pretreatment on the transcriptome of ox-LDL injured endothelial cells

ORGANISM(S): Homo sapiens

PROVIDER: GSE167392 | GEO | 2024/02/24

REPOSITORIES: GEO

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High-throughput Sequencing Facilitates Quantitative Analysis of Transcriptome Difference Underlying Quercetin Antioxidant Action in Ox-LDL-induced HUVECs Damage [ncRNA-Seq]

Project description:High-throughput Sequencing has revolutionized system-based analysis of cellular pathways. The goals of this study are to find the effect of quercetin pretreatment on the transcriptome of ox-LDL injured endothelial cells

2024-02-24 | GSE167391 | GEO

High-throughput Sequencing Facilitates Quantitative Analysis of Transcriptome Difference Underlying Quercetin Antioxidant Action in Ox-LDL-induced HUVECs Damage

Project description:High-throughput Sequencing Facilitates Quantitative Analysis of Transcriptome Difference Underlying Quercetin Antioxidant Action in Ox-LDL-induced HUVECs Damage

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Quercetin attenuates atherosclerosis via modulating oxidized LDL-induced endothelial cellular senescence

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2019-10-29 | GSE139288 | GEO

Expression data from ARPE-19 cells treated with LDL or ox-LDL

Project description:LDL or Ox-LDL 200ug/ml, which showed no loss of viability after a 48 hour exposure, induced a physiological and pathological transcriptional response, respectively. LDL induced a downregulation of genes associated with cholesterol biosynthesis while ox-LDL induced transcriptional alterations in genes related to inflammation, matrix expansion, lipid metabolism and processing, and apoptosis. Pentraxin-3 was secreted into the culture medium after RPE cells were stimulated with ox-LDL, and immunohistochemically evident in Bruchs membrane of human macular samples with age-related macular degeneration. ARPE-19 cells exposed to 200ug/ml ox-LDL had a 38% apoptosis rate compared to less than 1% when exposed to LDL or untreated controls (p<0.0001). While LDL induced a physiologic response by RPE cells, a pathological phenotypic response was seen after treatment with oxidatively modified LDL. The transcriptional, biochemical, and functional data provide initial support of a role for the hypothesis that modified LDLs are one trigger for initiating events that contribute to the development of age-related macular degeneration. Keywords: treatment with non-treatment control Human ARPE-19 cells were exposed to LDL or oxidatively modified LDL (ox-LDL) for 48 hours for RNA extraction and hybridization on Affymetrix microarrays. We sought to determine whether retina, pigment epithelial cells develop a pathologic phenotype after exposure to low density lipoproteins (LDL) that are oxidatively modified.We have made two comparsions: LDL treatment versus non-treatment; ox-LDL treatment versus non-treatment.

2006-09-01 | E-GEOD-5741 | biostudies-arrayexpress

Expression data from ARPE-19 cells treated with LDL or ox-LDL

Project description:LDL or Ox-LDL 200ug/ml, which showed no loss of viability after a 48 hour exposure, induced a physiological and pathological transcriptional response, respectively. LDL induced a downregulation of genes associated with cholesterol biosynthesis while ox-LDL induced transcriptional alterations in genes related to inflammation, matrix expansion, lipid metabolism and processing, and apoptosis. Pentraxin-3 was secreted into the culture medium after RPE cells were stimulated with ox-LDL, and immunohistochemically evident in Bruch’s membrane of human macular samples with age-related macular degeneration. ARPE-19 cells exposed to 200?g/ml ox-LDL had a 38% apoptosis rate compared to less than 1% when exposed to LDL or untreated controls (p<0.0001). While LDL induced a physiologic response by RPE cells, a pathological phenotypic response was seen after treatment with oxidatively modified LDL. The transcriptional, biochemical, and functional data provide initial support of a role for the hypothesis that modified LDLs are one trigger for initiating events that contribute to the development of age-related macular degeneration. Keywords: treatment with non-treatment control

2006-09-01 | GSE5741 | GEO

High-throughput Sequencing Facilitates Quantitative Analysis of Transcriptome Difference Underlying Quercetin Antioxidant Action in Ox-LDL-induced HUVECs Damage [miRNA-Seq]

Project description:High-throughput Sequencing Facilitates Quantitative Analysis of Transcriptome Difference Underlying Quercetin Antioxidant Action in Ox-LDL-induced HUVECs Damage [miRNA-Seq]

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High-throughput Sequencing Facilitates Quantitative Analysis of Transcriptome Difference Underlying Quercetin Antioxidant Action in Ox-LDL-induced HUVECs Damage [ncRNA-Seq]

Project description:High-throughput Sequencing Facilitates Quantitative Analysis of Transcriptome Difference Underlying Quercetin Antioxidant Action in Ox-LDL-induced HUVECs Damage [ncRNA-Seq]

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Expression data of human vascular smooth muscle cells (hVSMC) in response to n-LDL and ox-LDL.

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