Transcriptomics

Dataset Information

0

Comparison of WT vs. MHCII KO CD8 T cell transcriptomes from lungs of Mycobacterium tuberculosis infected mice


ABSTRACT: CD4 T cells are essential for immunity to tuberculosis because they produce cytokines including interferon-γ. Whether CD4 T cells act as “helper” cells to promote optimal CD8 T cell responses during Mycobacterium tuberculosis (Mtb) is unknown. We compared transcriptomes of purified lung CD8 T cells from Mtb infected WT and MHCII KO mice. Using two independent models, we validated RNA-seq results and showed that CD4 T cell help enhanced CD8 effector functions and prevented CD8 T cell exhaustion. We demonstrated synergy between CD4 and CD8 T cells in promoting the survival of infected mice. Purified helped, but not helpless, CD8 T cells efficiently restricted intracellular bacterial growth in vitro. Thus, CD4 T cell help plays an essential role in generating protective CD8 T cell responses against M. tuberculosis infection in vitro and in vivo. We infer vaccines that elicit both CD4 and CD8 T cells are more likely to be successful than vaccines that elicit only CD4 or CD8 T cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE167439 | GEO | 2021/02/25

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2023-04-24 | PXD037837 | Pride
2016-06-01 | E-GEOD-73118 | biostudies-arrayexpress
2018-10-26 | PXD005765 | Pride
2016-06-01 | GSE73118 | GEO
2011-08-22 | E-GEOD-30962 | biostudies-arrayexpress
2023-05-15 | GSE227851 | GEO
2023-03-22 | GSE227653 | GEO
2023-03-24 | GSE228113 | GEO
2016-07-01 | E-GEOD-73001 | biostudies-arrayexpress
2018-07-10 | GSE116830 | GEO