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Systematic Phenotyping and Characterization of the 5xFAD mouse model of Alzheimer’s Disease

ABSTRACT: we conducted a detailed phenotypic characterization of the 5xFAD model on a congenic C57BL/6J strain background, across its lifespan – including a seldomly analyzed 18-month old time point to provide temporally correlated phenotyping of this model and a template for characterization of new models of LOAD as they are generated. This comprehensive analysis included quantification of plaque burden, Aβ biochemical levels, and neuropathology, neurophysiological measurements and behavioral and cognitive assessments, and evaluation of microglia, astrocytes, and neurons. Analysis of transcriptional changes was conducted using bulk-tissue generated RNA-seq data from microdissected cortices and hippocampi as a function of aging, which can be explored at the UCI Mouse Explorer and AD Knowledge Portal. This deep-phenotyping pipeline identified novel aspects of age-related pathology in the 5xFAD model.

ORGANISM(S): Mus musculus

PROVIDER: GSE168137 | GEO | 2021/03/04

REPOSITORIES: GEO

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Systematic Phenotyping and Characterization of the 5xFAD mouse model of Alzheimer’s Disease

Project description:Systematic Phenotyping and Characterization of the 5xFAD mouse model of Alzheimer’s Disease

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