Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MiRNA expression data of spent in-vitro culture media condtioned with 2-cell, 8-cell, and blastocyst staged bovine embryos

ABSTRACT: Distinct miRNA populations have been detected in the spent media (SM) of in-vitro cultured embryos. However, profiling has only been conducted in SM cultured with blastocyst-stage embryos. Therefore, the aim of the study was to globally profile the extracellular microRNA (miRNA) population throughout the pre-implantation period in bovine embryos using a heterologous miRNA microarray

ORGANISM(S): synthetic construct Bos taurus

PROVIDER: GSE168551 | GEO | 2021/03/10

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

E. coli cells: Control vs. P. aeruginosa spent-media treated

Project description:Transcriptional profiling of E. coli cells comparing control untreated cells with cells treated with P. aeruginosa spent media. The goal was to identify the effects of P. aeruginosa spent media on E. coli cells.

2016-05-24 | GSE72182 | GEO

miRNA expression data of spent in-vitro culture media condtioned with 2-cell, 8-cell, and blastocyst staged bovine embryos

Project description:miRNA expression data of spent in-vitro culture media condtioned with 2-cell, 8-cell, and blastocyst staged bovine embryos

| PRJNA708283 | ENA

E. coli cells: Control vs. iron-supplemented P. aeruginosa spent-media treated

Project description:Transcriptional profiling of E. coli cells comparing control untreated cells with cells treated with iron-supplemented P. aeruginosa spent media. The goal was to identify the effects of P. aeruginosa spent media on E. coli cells, while neutralizing any effects of the P. aeruginosa siderophores

2016-05-24 | GSE72282 | GEO

Comprehensive analysis of soluble RNAs in human embryo culture media

Project description:Spent culture media was collected on day 5 after in vitro fertilization from 5 human blastocysts that were subsequently transferred or frozen. Corresponding blank culture media from the same lot were cultured alongside the embryos and used as negative controls along with PBS. The samples were prepared for total RNA sequencing using SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara) and for small RNA sequencing using the QIAseq RNA Library Prep kit (Qiagen). Prepared libraries were sequenced as 100 bp paired end on an Illumina HiSeq 4000 sequencer for total RNA and Illumina NextSeq500 sequencer for small RNA. We found that total RNA mapped only 3% of the reads to gene regions in spent culture media and control and only 2% in PBS samples mapped to gene regions. For the small RNA analysis, human annotated miRNAs constituted from 0.1% to 1.4% of the mapped reads and 40% of small RNA reads mapped to the human genome. tiRNAs derived from 5 different mature tiRNA were differentially expressed when comparing conditioned to unconditioned media. We conclude, that in spite of applying state-of-the-art sensitive detection methods no miRNAs were found to be reliably present in the spent culture medium. In contrast, tiRNA fragments appear to be overexpressed in cultured IVF media samples.

2020-09-07 | GSE142380 | GEO

Proteomic Profiling of Barley Spent Grains

Project description:Proteomic profiling was performed on barley spent grain. Spent grain from malted barley and from raw barley supplemented with exogenous enzymes were profiled.

2018-03-23 | PXD008090 | Pride

Characterization of microRNAs in spent culture medium associated with human embryo quality and development

Project description:Purpose: Given implantation failure limited the improvement of the in vitro fertilization (IVF) success rate, it was urgent to identify potential biomarkers for embryo quality and predicting the outcomes of IVF-ET. Methods: The expression profiles of 16 spent culture media (SCM) collected from embryos at the cleavage on Day 3 (D3 cleavage) and blastocyst stages on Day 5 (D5 blastocyst) during IVF cycles were determined with RNA-sequencing. Differentially expressed miRNAs (DEmiRNAs) were identified with p-value < 0.05 and |log2FC|> 1. Then, the miRNA-mRNA interaction networks were constructed by using Cytoscape software. Finally, the GO and KEGG pathway analysis of target genes of DEmiRNAs were conducted. Results: Compared with pregnant groups, 29 DEmiRNA were detected in non-pregnant groups at D3 cleavage, and 26 DEmiRNA were detected in non-pregnant group at D5 blastocyst. Among them, a total of six known miRNAs, including hsa-miR-199a-3p>hsa-miR-199b-3p, hsa-miR-199a-5p, hsa-miR-379-5p, hsa-miR-432-5p, hsa-miR-99a-5p and hsa-miR-483-5p, were identified. The results of GO and KEGG pathway analysis indicated that these target genes of DEmiRNAs were associated with various biological processes. Conclusion: In conclusion, we identified three miRNAs, including hsa-miR-199a-5p, hsa-miR-483-5p and hsa-miR-432-5p, may serve as biomarkers for embryo quality during IVF cycles.

2023-10-31 | GSE167961 | GEO

MicroRNA expression patterns in mouse tissues and staged embryos

Project description:RNA from seven adult mouse tissues, four staged whole mouse embryos, ES cells, 3 day and 28 day embryoid bodies was profiled against the microRNA microarray. A custom microarray platform for analysis of microRNA gene expression; Nature Methods 1, 47 - 53 (2004) Keywords = microRNA Keywords = miRNA Keywords: other

2004-09-02 | GSE1635 | GEO

MicroRNA expression patterns in mouse tissues and staged embryos

2010-10-08 | E-GEOD-1635 | biostudies-arrayexpress

Brewer’s spent dry yeast modulates immunity in gilthead sea bream (Sparus aurata)

Project description:We report a comparison of tissue-specific (head kidney, intestine and liver) gene expression profiles from gilthead sea bream fed with control and Brewer's spent dry yeast (SDY) diets.The inclusion of SDY at 30% in the experimental diet (40% crude protein, 16% crude lipid) resulted in a reduction in FM (10%) and PP (31.4%) contents. 218 differentially expressed genes (DEGs) were identified among all tissues, out of which, 141 were up- and 77 down-regulated. The enrichment analysis of DEGs revealed that SDY had a modulatory effect on several processes related to host’s immunity, oxygen’s carrier capacity, sexual differentiation, metabolism, and digestion. This study supports the notion that brewery’s by-products like SDY are suitable for aquafeeds of carnivorous fish species such as the gilthead sea bream, and promotes a circular bioeconomy model that reuses, recovers and recycles resources instead of producing wastes

2022-03-09 | GSE194206 | GEO

Transcriptional profiling of Bone Marrow Derived Dendritic Cells (BMDC) stimulated with zymosan or bacterial spent medium

Project description:We reported transcriptional characterization of BMDCs stimulated by filter-sterilized spent medium of Parabacteroides goldsteinii ASF519 (ASF519 SSM) alone or in comination with zymosan. We identified that ASF519 SSM-induced differentially expressed genes have a wide association with the regulation of immune functions, cell communication, apoptosis. We observed that ASF519 SSM induced transcriptomic changes towards immune tolerance, such as a dramatic induction of tolerogenic Il10, lipocalin-2(Lcn2) and indoleamine 2, 3-dioxygenases (Ido1/2), and a reduction of the co-stimulatory molecule Cd40 and inflammatory cytokines, including Il12b, Il23a, and Il1f9 (or Il-36 gamma).

2021-04-07 | GSE171658 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data