Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptomics of Kidney Transplant Recipients after Cytomegalovirus DNAemia

ABSTRACT: To study the gene expression dynamics in cytomegalovirus (CMV) DNAemic patients after kidney transplantation, we performed RNA sequencing (RNAseq) on 31 DNAemic patients at 4 timepoints (baseline, 1 week post-DNAemia, 1 month post-DNAemia, and a long-term timepoint 9-18 months post-DNAemia) matched with 31 non-DNAemic patients at 2 timepoints (baseline and long-term).

ORGANISM(S): Homo sapiens

PROVIDER: GSE168598 | GEO | 2021/11/04

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

PERFORMANCE OF STOOL AND SERUM CYTOMEGALOVIRUS REAL-TIME POLYMERASE CHAIN REACTION AND THEIR COMBINATION IN DIAGNOSING CMV COLITIS

Project description:Interventions: Patients with the detection of either cytomegalic cells or IHC-CMV cells were diagnosed with CMV colitis.,Patients older than 18 years with clinical suspicion of CMV colitis but negative biopsy for CMV.,Asymptomatic patients underwent colonoscopy for screening.;Diagnostic,Diagnostic,Diagnostic;CMV colitis,Non-CMV colitis ,Healthy volunteers Primary outcome(s): Diagnostic performance After colonoscopy Sensitivity, Specificity

| 2756739 | ecrin-mdr-crc

Transcriptome modulation analysis of cytomegalovirus-infected immature monocyte-derived dendritic cells

Project description:Analysis of dendritic cells (DCs) at 6h and 48h after infection with cytomegalovirus (CMV) versus non infected. CMV tends to induce immunosuppression followed by lasting immunity. DCs appear to play a role in this effect. Results provide insights into CMV/DC interactions and suggest mechanisms for CMV-regulated early immune response. Keywords: gene expression array-based, count In the present study, immature monocyte-derived dendritic cells were generated from CMV neg healthy blood donors and infected or not with an endotheliotropic CMV strain. Gene expression modulation by the CMV infection was studied in a time course assay (non-infected - NI - vs 6h or 48h post-infection. Patient C has no 48h sample

2009-01-22 | E-GEOD-14490 | biostudies-arrayexpress

DNA methylation profiling of lung transplant recipients

Project description:Targeted bisulfite sequencing data of peripheral blood mononuclear cells (PBMCs) from participants pre- and post- lung transplant(Tx) was generated to study 1) methylation difference between pre- and postTx, 2) methylation difference between cytomegalovirus (CMV) seropositive and seronegative in preTx, and 3) immune response regarding Tx and immunosuppression prophylaxes.

2024-10-15 | GSE253562 | GEO

DNA methylation profiling of kidney transplant recipients

Project description:Targeted bisulfite sequencing data of peripheral blood mononuclear cells (PBMCs) from participants pre- and post- kidney transplant(Tx) was generated to study 1) methylation difference between pre- and postTx, 2) methylation difference between cytomegalovirus (CMV) seropositive and seronegative in preTx, 3) immune response regarding Tx and immunosuppression prophylaxes, and 4) how the gene expression and DNA methylation are associated.

2024-10-15 | GSE250535 | GEO

Genetic variants and cell-specific signatures in PBMCs of pre transplant recipients

Project description:Long-term survival of the renal allograft is a challenge. A single cell sequencing analysis is a powerful approach to identify genetic/genomic risk for development of long-term graft loss at single cell level. In this study, we compared single cell RNA sequencing profiles of pre-transplant PBMC samples from 4 transplant patients (2 patients with graft loss within 3 years vs 2 patients without graft loss) with simultaneous genome-wide genotype data for common variants to idenitfy the immune cell subtypes and transcriptomic signatures associated with long-term graft loss. These transcriptomic data also integrates with genetic data in an effort to identify genes that mediate the risk for graft survival in a cell-type specific context.

2021-08-29 | GSE182916 | GEO

mRNA Expression Data from murine Medial Prefrontal Cortex and Hippocampus at baseline, 64 hours post-MI, and 8 weeks post-MI in Clock∆19/∆19 and WT C57Bl/6 mice

Project description:Male Clock∆19/∆19 and WT C57Bl/6 mice were randomized into 3 groups: i) baseline, ii) short-term post-myocardial infarction (MI; 64h post-MI), iii) long-term post-MI (heart failure, HF; 8 weeks post-MI). Medial prefrontal cortex (mPFC) and hippocampus brain regions were collected at ZT19 (waketime). The microarray approach allows the investigation of transcriptome-wide gene expression changes in brain regions from Clock∆19/∆19 and WT mice at baseline, post-MI, and in HF.

2019-01-05 | GSE124670 | GEO

Antigen-specific T-cell receptor signatures of cytomegalovirus infection

Project description:We immunosequenced peptide-stimulated PBMCs from 8 CMV-positive donors to identify virus-specific T cell receptors. We also sequenced ex vivo T cell repertoires of multiple CMV-postive and CMV-negative donors to compare the availability of CMV-specific TCRs in virus carriers and uninfected subjects.

2018-11-21 | GSE114931 | GEO

Transcriptomic profiling of kidney transplant recipients [RNA-seq]

Project description:RNA-seq data of peripheral blood mononuclear cells (PBMCs) from participants pre- and post- kidney transplant(Tx) was generated to study 1) gene expression difference between pre- and postTx, 2) gene expression difference between cytomegalovirus (CMV) seropositive and seronegative in preTx, 3) immune response regarding Tx and immunosuppression prophylaxes, and 4) how the gene expression and DNA methylation are associated.

2024-10-15 | GSE250426 | GEO

Competition but not cross-competition controls T cell proliferation in transplant patients following cytomegalovirus reactivation

Project description:Competition between antigen-specific T cells for peptide:MHC (p:MHC) complexes shapes the ensuing T cell response. T cells also compete for access to antigen presenting cells (APCs), which results in competition between T cells of different antigen specificities and is referred to as cross-competition. When both mechanisms are in place, a memory T cell population specific for just a single epitope can effectively limit de novo antigen-specific T cell responses. Whether human cytomegalovirus (CMV)-specific T cell responses prevent other de novo T cell responses is unknown, but of interest in a transplant context as well as the development of CMV-based vaccines. We assessed the extent of T cell competition and cross-competition in a cohort of non-myeloablative hematopoietic stem cell transplant (nmHSCT) patients. In our cohort, HSCT donors and recipients were CMV-seronegative and -positive, respectively, thus providing genetically distinct memory and naïve T cell populations. We used single-cell transcriptomics to track donor versus recipient-derived T cell clones. We found that recipient-derived CMV-specific memory T cells outcompeted donor-derived responses of identical specificity; however, expansion of other donor-derived T cell clones still occurred averaging 24 rounds of cell division. The transcriptional programs between expanding donor and recipient-derived cells were largely congruent and consistent with an effector T cell response. Our data further indicated that the composition of the T cell compartment changed due to expanding and contracting clones in the first 90 days post-nmHSCT. Overall, our data suggest that CMV-specific memory T cells compete for p:MHC but not APC access during CMV reactivation.

2022-01-18 | GSE167825 | GEO

Transcriptome modulation analysis of cytomegalovirus-infected immature monocyte-derived dendritic cells

2009-01-22 | GSE14490 | GEO