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Comparison of gene expression profiles between SCD1/FADS2 knockdown and vector control of ovarian cancer cells OVCA433 by RNA-sequencing

ABSTRACT: To understand the dependence of ovarian tumor cells on fatty acid metabolism, we performed transcriptome analysis using OVCA433 ovarian cancer cells, in which SCD1/FADS2 was genetically ablated via CRISPER/Cas9.

ORGANISM(S): Homo sapiens

PROVIDER: GSE168720 | GEO | 2021/03/12

REPOSITORIES: GEO

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Comparison of gene expression profiles between SCD1/FADS2 knockdown and vector control of ovarian cancer cells OVCA433 by RNA-sequencing

Project description:Comparison of gene expression profiles between SCD1/FADS2 knockdown and vector control of ovarian cancer cells OVCA433 by RNA-sequencing

| PRJNA713769 | ENA

Expression data in HCT-116 colon cancer cell treated with SCD1 inhibitor or in SCD1 knocked out HCT-116 cell

Project description:To understand molecular mechanisms underlying the growth inhibitory ativity of Stearoyl-CoA desaturase-1 (SCD1) inhibitor, we performed microarray analysis using HCT-116 colorectal cancer cells, in which SCD1 was pharmacologically blocked or genetically ablated.

2017-08-01 | GSE98364 | GEO

Comparison of gene expression profiles between SCD1 knockdown and vector control of Huh7 cells and PLC/PRF/5 cells by RNA-sequencing

Project description:The purpose of this experiment is to search for the downstream target of SCD1

2017-09-20 | GSE86602 | GEO

SCD1 Modulates the Skin Homeostasis via Sustaining Hemidesmosomes

Project description:Niche for stem cells can profoundly influence the position and fate of stem cells in tissue homeostasis and associated disorders, however, the mechanisms underlying the maintenance of stem cell niche remain poorly understood. Here we report that fatty acid desaturation catabolized by stearoyl-coenzyme A desaturase 1 (SCD1) regulates the activity of hair follicle stem cells (HFSCs) via blocking the formation of the bulge, a niche for HFSCs. We further unraveled that the abnormal hair growth in mice with SCD1 deletion is due to its function in K14+ basal keratinocytes rather than directly affecting HFSCs. Mechanistically, SCD1 deficiency impaired the expression of integrin α6 and the formation of hemidesmosomes (HDs), and thus allowed the activation of FAK in K14+ keratinocytes. Activated FAK subsequently stimulated PI3K and promoted the differentiation and proliferation of these keratinocytes, resulting in downward extension of the outer root sheath (ORS) and the disruption of bulge formation. Interestingly, inhibition of PI3K signaling restored bulge formation and normalized HFSCs and hair growth. Supplementation of oleic acid, the product of SCD1, to SCD1-/- mice also enhanced HD numbers, normalized the niche of HFSCs, and promoted hair growth in SCD1-/- mice. Taken together, SCD1 is indispensable for hair growth through stabilizing the number of HDs and thus sustaining bulge formation for HFSC residence and periodic activity.

2023-02-08 | GSE184346 | GEO

Scd1 Knockout Mice on very low fat diet

Project description:Stearoyl-CoA desaturase 1-deficient (SCD1-/-) mice have impaired monounsaturated fatty acid (MUFA) synthesis. When maintained on a very low-fat, high-carbohydrate (VLF-HC) diet, SCD1-/- mice develop severe hypercholesterolemia characterized by an increase in apolipoprotein B-containing lipoproteins and the appearance of lipoprotein-X. Additionally, high-density lipoprotein cholesterol is dramatically reduced in VLF-HC SCD1-/- mice. The concomitant presence of elevated plasma bile acids, bilirubin and aminotransferases in the VLF-HC SCD1-/- mouse are indicative of hepatic dysfunction. Supplementation of the VLF-HC diet with unsaturated fat (canola oil), but not saturated fat (coconut oil), prevents these plasma phenotypes. However, dietary oleate was not as effective as canola oil in reducing low-density lipoprotein cholesterol, signifying an additional role for dietary polyunsaturated fatty acid deficiency in the development of this phenotype. These results indicate that lack of SCD1 results in an increased requirement for dietary unsaturated fat to compensate for impaired MUFA synthesis and to prevent hypercholesterolemia and hepatic dysfunction. Keywords: repeat (genotype and diet)

2005-12-23 | GSE3889 | GEO

Escherichia coli SCD1

Project description:Escherichia coli SCD1 Genome sequencing and assembly

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Expression data of A939572 SCD1 inhibitor treated ccRCC cells

2012-10-11 | GSE41485 | GEO

Expression data of A939572 SCD1 inhibitor treated ccRCC cells

Project description:Presently, there is a deficiency of effective therapies designed to target clear cell renal cell carcinoma (ccRCC), with poor prognosis resulting in patients with advanced disease. Additionally, there is a lack of molecular factors which can be remedially targeted resulting in tumor specific inhibition, and therefore current therapeutic approaches often produce adverse side effects in patients. We identified that Stearoyl-CoA desaturase 1 (SCD1) was consistently overexpressed in patient ccRCC samples, and further investigation of SCD1 as a potential molecular target for ccRCC intervention utilizing a SCD1 inhibitor (A939572) resulted in tumor specific growth inhibition and induction of cell death. In order to understand the mechanism by which the SCD1 inhibitor mediated its anti-tumor effects, we performed gene array analysis and compared expression patterns between treated and untreated samples. Four established ccRCC cell lines purchased from ATCC were treated with DMSO control versus A939572 over a 24 hour time period. RNA from each group was isolated and sent of for gene array anaylsis. Gene expression data from each individual treatment group was normalized to its respective DMSO contol, and resulting expression patterns associated with drug exposure was compared between the four cell lines.

2012-10-11 | E-GEOD-41485 | biostudies-arrayexpress

RNA sequencing analysis of wild-type and Scd1-deficient CD4+ early iTregs

Project description:Analysis of differentially expressed genes in CD4+ early iTregs isolated from wild-type and Scd1-deficient mice. The goal of this study was to elucidate the effect of Scd1 on Treg differentation.

2023-02-13 | GSE224839 | GEO

RNA sequencing analysis of wild-type and Scd1-deficient CD4+ naïve T cells

Project description:Analysis of differentially expressed genes in Scd1-deficient CD4+ naïve T cells. The goal of this study was to elucidate the underlying pathway of Scd1-deficiency leading to increased regulatory T cell differentiation.

2023-08-28 | GSE160040 | GEO

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