Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next Generation Sequencing Facilitates Quantitative Analysis of Honey Bee Brain Transcriptomes Treated by Dinotefuran

ABSTRACT: Purpose：The goals of this study are to obtain NGS-derived honey bee brain transcriptome profiling (RNA-seq) after dinotefuran treatment in honey bee Methods：Through deep sequencing in triplicate, using Illumina Hiseq2500,a comprehensive analysis of lncRNAs and mRNAs was performed following persistent exposure to 0.01 mg/L dinotefuran for 1, 5, and 10 d. Results： In total, 312 lncRNAs and 1341 mRNAs, 347 lncRNAs and 1458 mRNAs, 345 lncRNAs and 1155 mRNAs were found to be differentially expressed (DE) on days 1, 5 and 10 respectively. Conlusions：This study characterized the expression profile of lncRNAs upon exposure to neonicotinoid insecticides in young adult honey bees.

ORGANISM(S): Apis mellifera

PROVIDER: GSE168740 | GEO | 2021/07/07

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Searching beyond the streetlight: neonicotinoid exposure alters the neurogenomic state of worker honey bees

Project description:Neonicotinoid insecticides have been implicated in honey bee declines, with many studies showing that sub-lethal exposure impacts bee behaviors such as foraging, learning and memory. Despite the large number of ecotoxicological studies carried out to date, most focus on a handful of worker phenotypes leading to a ‘streetlight effect’ where the a priori choice of phenotypes to measure may influence the results and conclusions arising from the studies. This bias can be overcome with the use of toxicological transcriptomics, where changes in gene expression can provide a more objective view of how pesticides alter animal physiology. Here, we used RNA sequencing to examine the changes in neurogenomic states of nurse and forager honey bees that were naturally exposed to neonicotinoids in the field and artificially exposed to neonicotinoid in a controlled experiment.

2022-01-04 | GSE178742 | GEO

Genetics of tolerance in honey bees to the neonicotinoid clothianidin.

Project description:The effects of neonicotinoid insecticides (NNIs) on honey bee health is intensely debated, with numerous studies showing negative effects of exposure, while others report no such effects. Understanding the cause of these differences is critical for developing evidence-based policy on the use of NNIs. We carried out experiments to study the genetic and molecular basis of NNI tolerance in honey bees, which may underlie the discrepancies observed in the literature. We discovered that worker survival post-exposure to an acute oral dose of clothianidin is heritable (H2=37.8%).

2023-03-01 | GSE216021 | GEO

Genome-wide analysis of coding and non-coding RNAs in ovary of honey bee workers

Project description:The present study is the first study to identify the involvement of mRNA, lncRNAs, circRNAs and miRNA in the ovary of honey-bee workers.We predicted 10271 mRNAs, 7235 lncRNAs, 11794 circRNAs and 164 miRNAs in the ovary of honey bee workers.

2019-08-01 | GSE119256 | GEO

The IRE1 Pathway Regulates Honey Bee Unfolded Protein Response Gene Expression

Project description:Our molecular understanding of honey bee cellular stress responses is incomplete. Previously, we sought to identify and began functional characterization of the components of the UPR in honey bees. We observed that UPR stimulation resulted in induction of target genes upon and IRE1 pathway activation, as assessed by splicing of Xbp1 mRNA. However, were not able to determine the relative role of the various UPR pathways in gene activation. Our understanding of honey bee signal transduction and transcriptional regulation has been hampered by a lack of tools. After using RNAseq to expand the known UPR targets in the bee, we use the Drosophila melanogaster S2 cell line and honey bee trans and cis elements to investigate the role of the IRE-1 pathway in the transcriptional activation of one of these targets, the honey bee Hsc70-3 gene. Using a luciferase reporter, we show that honey bee hsc70 promoter activity is inducible by UPR activation. In addition, we show that this activation is IRE1-dependent and relies on specific cis regulatory elements. Experiments using exogenous honey bee or fruit fly XBP1S proteins demonstrate that both factors can activate the Hsc70-3 promoter and further support a role for the IRE-1 pathway in control of its expression in the honey bee. By providing foundational knowledge about the UPR in the honey bee and demonstrating the usefulness of a heterologous cell line for molecular characterization of honey bee pathways, this work stands to improve our understanding of this critical species.

2020-03-28 | GSE139368 | GEO

Single-cell dissection of aggression in honeybee colonies

Project description:RNA-Sequencing performed on 177 honey bee whole-brains, divided into "soldier" and "forager" groups from Puerto Rican honey bee colonies.

2023-05-09 | GSE146085 | GEO

Intronic Non-CG DNA Hydroxymethylation and Alternative mRNA Splicing in Honey Bees

Project description:Honey bee non-CG DNA hydroxymethylation is enriched in the introns, which supplements previous findings that honey bee CG DNA methylation is enriched in exons.

2013-09-19 | GSE50990 | GEO

Abundant small RNAs in the reproductive tissues of the honey bee, Apis mellifera, are a plausible mechanism for epigenetic inheritance and parental manipulation of gene expression

Project description:Here we present the first characterisation of small RNAs in honey bee reproductive tissues. We conclude that small RNAs are likely to play an integral role in honey bee gametogenesis and reproduction and provide a plausible mechanism for parent-of origin-effects on gene expression and reproductive physiology. present in honey bee reproductive tissues: ovaries, spermatheca, semen, fertilised and unfertilised eggs, and testes.

2022-04-13 | GSE182720 | GEO

The habitat disruption induces immune-suppression and oxidative stress in honey bees

Project description:Background: Honey bee is a major insect used for pollination of a number of commercial crops worldwide. However, the number of managed honey bee colonies has recently declined in several countries, and a number of possible causes are proposed. Although the use of honey bees for pollination can be considered as disruption of the habitat, its effects on honey bees' physiology have never been addressed. In Japan, more than 100 thousands colonies are annually used for pollination, and intriguingly 80% of them are used in greenhouses. Recently, honey bee colonies have often collapsed when they are introduced into greenhouses. Thus, to suppress colony collapses and maintain the number of worker bees in the colonies are essential for successful long-term pollination in greenhouses and recycling honey bee colonies.

2011-05-12 | GSE29252 | GEO

Transcriptome of honey bee larvae

Project description:Honey bee drones, queens and workers have vastly different phenotypes. Here we profile the the expression level of mRNAs and microRNAs of honeybee, drones, queens and workers at the L5 larval stage (91 hours +/- 1).

2015-09-06 | GSE61253 | GEO

Brain transcriptomics of honey bee parasitized by Varroa destructor or Nosema ceranae

Project description:In this study we addressed whether the transcriptome profile in the honey bee brain is similar for two major parasites of honey bee, Varroa destructor and Nosema ceranae. Honey bees parasitized by these two parasites show accelerated behavioral maturation and deficiences in orientation and learning/memory that we hoped to characterized at the transcriptomic level.

2013-09-24 | GSE41109 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data