Transcriptomics

Dataset Information

0

Mesenchymal stromal cells improve the transplantation outcome of CRISPR-Cas9 gene-edited human HSPCs (RNA-seq)


ABSTRACT: Mesenchymal Stromal Cells (MSCs) have been employed in vitro to support HSPC expansion and in vivo to promote Hematopoietic Stem and Progenitor Cells (HSPCs) engraftment. Based on these studies, we developed an MSC-based co-culture system to optimize transplantation outcome of CRISPR-Cas9 gene-edited (GE) human HSPCs. We show that bone marrow (BM)-MSCs produce several hematopoietic supportive and anti-inflammatory factors capable to alleviate the proliferation arrest and mitigate the apoptotic and inflammatory programs activated in GE-HSPCs, improving their expansion and clonogenic potential in vitro. The use of BM-MSCs resulted in superior human engraftment and increased clonal output of GE-HSPCs contributing to the early phase of hematological reconstitution in the peripheral blood of transplanted mice. In conclusion, our work poses the biological bases for a novel clinical use of BM-MSCs to promote engraftment of GE-HSPCs and improve their transplantation outcome.

ORGANISM(S): Homo sapiens

PROVIDER: GSE168834 | GEO | 2022/09/01

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2022-09-01 | GSE206902 | GEO
2022-09-01 | GSE206900 | GEO
2020-06-22 | GSE113857 | GEO
2024-04-15 | PXD042257 | Pride
2024-01-17 | GSE253105 | GEO
2016-09-01 | E-GEOD-79091 | biostudies-arrayexpress
2023-06-02 | GSE220466 | GEO
2024-08-28 | GSE275808 | GEO
2017-11-01 | GSE90552 | GEO
2022-03-16 | PXD028481 | Pride