Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptomic analysis of porcine granulosa cells cocultured with COCs and exposed to the high level of NEFAs during IVM

ABSTRACT: The transcriptome pattern in cocultured granulosa cells that exposed to the high level of NEFAs duing IVM was investigated. Genome-wide transcriptome analysis was performed by microarray using EDMA platform.

ORGANISM(S): Sus scrofa

PROVIDER: GSE168957 | GEO | 2021/11/03

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcription analysis of blastocyst form oocyte exposed to the high level of NEFAs with cocultured of monolayer of GCs

Project description:The transcriptome pattern in blastocyst that developed from oocytes exposed to the high level of NEFAs with cocultured of monolayer of GSs was investigated. Genome-wide transcriptome analysis was performed by micro array using EDMA platform.

2022-07-20 | GSE154836 | GEO

Transcriptomic analysis of porcine granulosa cells cocultured with COCs and exposed to the high level of NEFAs during IVM

Project description:Transcriptomic analysis of porcine granulosa cells cocultured with COCs and exposed to the high level of NEFAs during IVM

| PRJNA714658 | ENA

DNA methylation analysis of blastocyst form oocyte exposed to the high level of NEFAs when cocultured with monolayer of GCs

Project description:The DNA methylation pattern in blastocyst that developed from oocytes exposed to the high level of NEFAs was investigated. Genome-wide DNA methylation analysis was performed by micro array using EDMA platform.

2022-07-20 | GSE154835 | GEO

Transcriptomic analysis of porcine blastocyst from oocyte exposed to the high level of NEFAs

Project description:The transcriptome pattern in blastocyst that developed from oocytes exposed to the high level of NEFAs was investigated. Genome-wide transcriptome analysis was performed by micro array using EDMA platform.

2022-07-07 | GSE154432 | GEO

DNA methylation analysis of porcine blastocyst from oocyte exposed to the high level of NEFAs

2022-07-07 | GSE154431 | GEO

Analysis of blastocyst form oocyte exposed to the high level of NEFAs with cocultured of monolayer of GCs

Project description:Analysis of blastocyst form oocyte exposed to the high level of NEFAs with cocultured of monolayer of GCs

| PRJNA647782 | ENA

Transcription analysis of blastocyst form oocyte exposed to the high level of NEFAs with cocultured of monolayer of GCs

Project description:Transcription analysis of blastocyst form oocyte exposed to the high level of NEFAs with cocultured of monolayer of GCs

| PRJNA707112 | ENA

DNA methylation analysis of blastocyst form oocyte exposed to the high level of NEFAs when cocultured with monolayer of GCs

Project description:DNA methylation analysis of blastocyst form oocyte exposed to the high level of NEFAs when cocultured with monolayer of GCs

| PRJNA860289 | ENA

NR5A1 and FOXL2 Induce Human Fibroblasts into Steroidogenic Ovarian Granulosa-Like Cells

Project description:Granulosa cells provide essential niche for ovarian folliculogenesis by communicating with oocytes and secreting steroids at different follicular stages, but current cellular models for studying human granulosa cells mainly rely on immortal cancer cell lines. Here, we established a reprogramming method to collect early and late granulosa cells with different steroidogenic abilities. We applied AMH-fluorescence-reporter system to screen six candidate factors for cellular reprogramming and generated human induced granulosa like cells (hiGC) by overexpressing FOXL2 and NR5A1. AMH-EGFP+ hiGC resembled human cumulus cells in transcriptome profiling and secreted high levels of estrogen as well as progesterone, similar to late-stage granulosa cells at antral or preovulatory stage. Moreover, we identified a cell surface marker CD55 which can be used to isolate early-stage granulosa cells. CD55+AMH-EGFP- hiGC secreted high levels of estrogen but low levels of progesterone, and the transcriptome profiles were more similar to early-stage granulosa cells. Both types of the hiGC promoted oocyte maturation when cocultured with immature oocytes. More importantly, when CD55+ hiGC were transplanted to mouse model with Polycystic ovary syndrome (PCOS), the number of cystic follicles was significantly reduced, showing alleviation of PCOS. Hence, hiGC provides a cellular model to study developmental program of human granulosa cells and has potential to treat PCOS.

2023-12-31 | GSE203070 | GEO

Cancer cells cocultured with macrophage

Project description:Cancer cells cocultured with macrophage

| PRJNA877107 | ENA