Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq analysis on the liver of darkness rats treated with Limosilactobacillus reuteri

ABSTRACT: We have previously demonstrated that the arrhythmic expressions of circadian clock genes due to constant darkness induce glycometabolic and reproductive hallmarks of polycystic ovary syndrome (PCOS) in rats. Limosilactobacillus reuteri (L.reuteri) is a promising dietary intervention for host dysmetabolism, while its potential effect on circadian dysrhythmia-induced PCOS remains elusive. Here, we evaluated the amelioration of L.reuteri regimen on constant darkness-induced PCOS-like rats through detecting hepatic gene expression profiles by RNA-seq.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE169501 | GEO | 2023/07/11

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Urinary bladder in constant darkness: time course

2012-05-01 | GSE35795 | GEO

Urinary bladder in constant darkness: time course

Project description:Analysis of urinary bladder in wild-type C57BL/6 females sacrificed every 4 hours at six time points under constant darkness after acclimation for 2 weeks under 12-hour light and 12-hour dark conditions. Results provide insight into circadian gene expression patterns in normal urinary bladder. Analysis of urinary bladder in wild-type C57BL/6 females sacrificed every 4 hours at six time points (n=2 for each time (CT 0, 4, 8, 12 and 20)) under constant darkness after acclimation for 2 weeks under 12-hour light and 12-hour dark conditions.

2012-04-30 | E-GEOD-35795 | biostudies-arrayexpress

RNA-seq analysis on the liver of darkness rats treated with Limosilactobacillus reuteri

Project description:RNA-seq analysis on the liver of darkness rats treated with Limosilactobacillus reuteri

| PRJNA716818 | ENA

Rhythmic growth explained by coincidence between internal and external cues

2007-06-24 | GSE6906 | GEO

Transcription profiling of aortae from mice that have been subjected to constant darkness to study the transcriptional responses at different time points.

Project description:After 2 week acclimation to 12 hr/12 hr light/dark regimen, C57/BL6 mice were subjected to a 36 hour period of constant darkness. Aortae were subsequently harvested at 4 hour intervals to 48 hours. Duplicate microarrays were hybridized with biotin-labeled probes derived from aorta tissue (6 pooled aortae/timepoint). Keywords = aorta, murine, circadian

2007-11-06 | E-GEOD-414 | biostudies-arrayexpress

Transcription profiling of Arabidopsis wild type and arrhythmic CCA1-34 line reveals that rhythmic growth is explained by coincidence between internal and external cues

Project description:Plant hypocotyls elongate in response to darkness. The response to darkness is gated by the circadian clock, such that wild-type plants (Col) only respond to darkness with growth once every 24 hours, whereas arrhythmic lines, such as CCA1-34, will respond to darkness with growth at any time of day. The experiment here was designed to find genes whose expression was correlated with growth. It should also pick up other genes that are gated by the circadian clock or that are direct targets of CCA1. Experiment Overall Design: Two genotypes were uses, wild-type (Col) and the arrhythmic CCA1-34 line that overexpresses the CCA1 protein. Plants were entrained in short day conditions for four days and then subjected light/dark pulses consisting of 160 minutes of light and 320 minutes of dark. We collected samples 120 minutes into the first and last dark periods of the day, corresponding to 280 and 1240 minutes after dawn. At the 280 minute time point Col plants are growing in response to darkness. At the 1240 minute time point Col plants are not growing. CCA1-34 plants are growing at both time points. The collection regime was continued for three days for a total of three temporal replicates for each time point/genotype combination.

2008-06-15 | E-GEOD-6906 | biostudies-arrayexpress

Defining the Independence of the Liver Circadian Clock

Project description:Mammals rely on a network of circadian clocks to control daily systemic metabolism and physiology. The central pacemaker in the suprachiasmatic nucleus (SCN) is considered hierarchically dominant over peripheral clocks, whose degree of independence, or tissue-level autonomy, has never been ascertained in vivo. Using arrhythmic Bmal1-null mice, we generated animals with reconstituted circadian expression of BMAL1 exclusively in the liver (Liver-RE). High-throughput transcriptomics and metabolomics show that the liver has independent circadian functions specific for metabolic processes such as the NAD+ salvage pathway and glycogen turnover. However, although BMAL1 occupies chromatin at most genomic targets in Liver-RE mice, circadian expression is restricted to ∼10% of normally rhythmic transcripts. Finally, rhythmic clock gene expression is lost in Liver-RE mice under constant darkness. Hence, full circadian function in the liver depends on signals emanating from other clocks, and light contributes to tissue-autonomous clock function.

2019-06-13 | GSE132659 | GEO

BCAA catabolic mutants in prolonged darkness

Project description:We used RNA-Seq to ask whether the transcripts for the proposed BCKDH subunits are upregulated in wild-type plants subjected to prolonged darkness. These experiments were performed using rosette leaves from 5-week-old, short-day-grown (8h light/16h dark) Col-0 wild-type plants moved to constant darkness for 6h, 24h, 48h and 72h, and grown in short day for 72h as control. The transcripts of eight BCAA catabolism genes were increased nine- to 400-fold within the first 6h of prolonged darkness, and remained high until the last time point. These results are consistent with the hypothesis that BCAA catabolic enzymes - including BCKDH subunits E1A1, E1B1, E1B2 and E2 - have one or more physiological roles in the dark.

2015-04-16 | GSE67956 | GEO

LD/DD time course of y w; tim01 Drosophila #2

Project description:Background. The transcriptional circuits of circadian clocks control physiological and behavioral rhythms. Light may affect such overt rhythms in two ways: (1) by entraining the clock circuits and (2) via clock-independent molecular pathways. In this study we examine the relationship between autonomous transcript oscillations and light-driven transcript responses Methodology/Principal Findings. Transcript profiles of wild-type and arrhythmic mutant Drosophila were recorded both in the presence of an environmental photocycle and in constant darkness. Systematic autonomous oscillations in the 12-48 hr period range were detectable only in wild-type flies and occurred preferentially at the circadian period length. However, an extensive program of light-driven expression was confirmed in arrhythmic mutant flies. Many lightresponsive transcripts are preferentially expressed in the adult compound eye and the phospholipase C component of phototransduction, NO RECEPTOR POTENTIAL (NORPA), is required for their light-dependent regulation. Conclusions/Significance. Although there is growing evidence for the existence of multiple molecular clock circuits in plants and fungi, Drosophila appears to possess only one such system. The sustained photic expression responses identified here are partially coupled to the circadian clock and may reflect a mechanism for flies to modulate functions such as visual sensitivity and synaptic transmission in response to seasonal changes in photoperiod. Keywords: Time course

2006-02-03 | GSE3832 | GEO

LD/DD time course of y w; tim01 Drosophila #1

2006-02-03 | GSE3831 | GEO