Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell transcriptome analysis of aging bone marrow

ABSTRACT: Single-cell transcriptome analysis of 1-month, 6-month, 20-month mouse bone marrow

ORGANISM(S): Mus musculus

PROVIDER: GSE169608 | GEO | 2022/11/18

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Single-cell transcriptome analysis of aging bone marrow

Project description:Single-cell transcriptome analysis of aging bone marrow

| PRJNA717178 | ENA

Single-cell transcriptome analysis of Terc KO bone marrow and brain

Project description:Single-cell transcriptome analysis of 6-month Terc KO bone marrow and brain

2022-11-18 | GSE190535 | GEO

Single-cell transcriptome analysis of aging brain

Project description:Single-cell transcriptome analysis of 1-month, 6-month, 20-month brain

2022-11-18 | GSE169606 | GEO

Single cell profiling of bone marrow adipocytes and bone marrow stromal cells from irradiated mice.

Project description:In order to comprehensively characterize bone marrow mesenchymal cells after myeloablation, single-nuclei RNA sequencing was performed on bone marrow adipocytes and bone marrow stromal cells isolated from sublethally-irradiated mice.

2023-07-31 | GSE227255 | GEO

Age of the bone marrow dictates clonality of smooth muscle-derived cells in the atherosclerotic plaque [bulk RNA-seq]

Project description:Purpose: The goal of this study is to investigate the gene expression in aged (18 month old) and young (3 month old) bone marrow-derived monocytes. Methods: Bone marrow was harvested by flushing the femurs and tibias of young (3 month old) and aged (18 month old) mice in PBS with 2% fetal bovine serum (FBS) on ice. Cells were isolated from bone marrow by density gradient centrifugation using Lympholyte (Cedarlane, CL0531). After centrifugation at 1300 g for 20 min, bone marrow cells were collected from the interface and washed in Ca2+/Mg2+ - free HBSS. The cells were dissociated to a single-cell suspension by filtering through a 70-µm nylon mesh. For monocyte isolation, a suspension of bone marrow cells was stained with Alexa 700 anti-CD3 (1:100, BioLegend, 100215), PE-Cy7 anti-CD19 (1:100, BioLegend, 115520), FITC anti-CD11b (1:100, BD Pharmingen, 553310) and APC anti-Ly6c antibodies (1:100, BioLegend, 128015) in PBS with 0.5% FBS for 30 min. CD11b+Ly6c+CD3-CD19- monocytes were isolated with a BD FACSAria cell sorter. Total RNA was extracted and subjected to bulk RNA-seq using Illumina HiSeq 2000. Results: Expression profiles of the transcriptome are compared between bone marrow-derived monocytes isolated from aged (18 month old) and young (3 month old) mice. Conclusions: Significant differences in the expression levels of genes are noted between the two age groups.

2022-08-01 | GSE206429 | GEO

Bone Marrow Transplantation in Treating Patients With Hematologic Cancer

Project description:RATIONALE: Radiation therapy uses high-energy x-rays to damage cancer cells. Drugs used in chemotherapy use different ways to stop cancer cells from dividing so they stop growing or die. Combining chemotherapy with bone marrow transplantation may allow the doctor to give higher doses of chemotherapy drugs and kill more tumor cells. PURPOSE: Phase II trial to study the effectiveness of bone marrow transplantation in treating patients who have hematologic cancer.

| 2004880 | ecrin-mdr-crc

Single cell transcriptome profiling of Reticular Dysgenesis patient bone marrow

Project description:Single cell transcriptome profiling of Reticular Dysgenesis patient bone marrow

| PRJNA743317 | ENA

Expression data from mouse bone marrow adipocytes with age

Project description:The aim of this study was to characterize the age-related gene expression profiles between bone marrow adipocytes and peripheral white adipocytes. Alterations of gene expression with aging were analyzed in bone marrow and peripheral white adipocytes isolated from C57BL/6J male mice using Affymetrix Mouse Gene 1.0 ST arrays. Bone marrow adipocytes and peripheral white adipocytes (n=6-10 animals per group) were isolated from male C57BL/6J mice (6-months, 14-months and 18-months of age). Samples were grouped into cell type (bone marrow adipocytes vs. peripheral adipocytes) and age (6-month (6M), 14-month (14M) and 18-month (18M)).

2011-11-29 | E-GEOD-25905 | biostudies-arrayexpress

Single-cell gene expression of control mandibular bone marrow cells and mandibular bone marrow cells under apical periodontitis

Project description:Single-cell gene expression of mandibular bone marrow cells and mandibular bone marrow cells under the stimulation of apical periodontitis were determined by scRNAseq.

2023-02-11 | GSE212975 | GEO

Single cell profiling of bone marrow adipocytes and bone marrow stromal cells from irradiated mice.

Project description:Single cell profiling of bone marrow adipocytes and bone marrow stromal cells from irradiated mice.

| PRJNA944245 | ENA