Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression in mature pollen and sperm cells versus young seedling as a vegetative sporophyte reference control

ABSTRACT: Sperm cells represent the male partner that fuses with the egg cell during fertilization in all multi-cellular eukaryotic organisms, and, in flowering plants, is a founder of both embryo and nutritive endosperm. We examined the transcriptome of Oryza sativa ssp. japonica using the Affymetrix 57K rice genome GeneChip to provide an overview of genes activated in the paternal gamete. We used microarrays to detail the global program of gene expression in mature pollen and sperm cells at anthesis, which constitutes the stage immediately preceding pollen germination and gamete deposition leading up to fertilization. This documents the condition of the male gametophytic cells prior to fertilization. Keywords: tissue expression profile, male gametophyte, sperm, pollen, seedling

ORGANISM(S): Oryza sativa

PROVIDER: GSE17002 | GEO | 2012/04/30

SECONDARY ACCESSION(S): PRJNA117739

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene expression in mature pollen and sperm cells versus young seedling as a vegetative sporophyte reference control

Project description:Sperm cells represent the male partner that fuses with the egg cell during fertilization in all multi-cellular eukaryotic organisms, and, in flowering plants, is a founder of both embryo and nutritive endosperm. We examined the transcriptome of Oryza sativa ssp. japonica using the Affymetrix 57K rice genome GeneChip to provide an overview of genes activated in the paternal gamete. We used microarrays to detail the global program of gene expression in mature pollen and sperm cells at anthesis, which constitutes the stage immediately preceding pollen germination and gamete deposition leading up to fertilization. This documents the condition of the male gametophytic cells prior to fertilization. Keywords: tissue expression profile, male gametophyte, sperm, pollen, seedling Male gametophytic cells consisting of isolated pollen and isolated sperm cells collected at anthesis were compared with seedlings containing leaf, stem, root and apical meristems as a vegetative sporophytic control; three separate biological replicates were used.

2012-04-29 | E-GEOD-17002 | biostudies-arrayexpress

Comparative gene expression in mature egg cell, fertilized egg cell (1-1.5 hap) and fertilized zygote (2.5-3 hap)

Project description:Egg cells represent the female partner that fuses with the sperm cell during fertilization in all multi-cellular eukaryotic organisms—and, in flowering plants, is a founder of the embryo. We examined the transcriptome of Oryza sativa ssp. japonica (cv Katy) using the Affymetrix 57K rice genome GeneChip to provide an overview of genes activated in the maternal gamete, transcripts transmitted by the paternal genome, and changes in transcript up- and down-regulation during fertilization. We used microarrays to detail the global program of gene expression in the egg cell at anthesis, complementing prior data on the male germ lineage, and following gene expression during fertilization and gamete fusion.

2022-12-31 | GSE27400 | GEO

Comparative gene expression in synergid cells and antipodal cells at anthesis

Project description:Synergid cells represent the receptor cell type of angiosperm gametophytes that attract and receive the male pollen tube and are implicated in the transfer of the sperm cells to the target egg and central cells prior to double fertilization. The antipodals represent an accessory cell type that typically degenerates after fertilization; in rice it may initially have a short-lived nutritional role prior to endosperm establishment. We examined the transcriptome of these two cell types using Oryza sativa ssp. japonica (cv Katy) using the Affymetrix 57K rice genome GeneChip to provide an overview of genes activated at receptivity in the synergid and antipodal cells and transcript up- and down-regulated at female gametophyte maturity just prior to fertilization. This is part of a larger microarray paroject to examine the global program of gene expression during sexual reproduction in Oryza sativa which serves as a model angiosperm in which male and female gametophytic and embryonic cells may be readily isolated and characterized.

2022-12-31 | GSE56108 | GEO

Oryza sativa egg, sperm, callus, pollen and seedling proteome

Project description:In angiosperms, female gamete differentiation, fertilization, and subsequent zygotic development occur in embryo sacs deeply embedded in the ovaries. Despite their importance in plant reproduction and development, how the egg cell is specialized, fuses with the sperm cell, and converts into an active zygote for early embryogenesis remains unclear. This lack of knowledge is partly attributable to the difficulty of direct analyses of gametes in angiosperms. In this study, proteins from egg and sperm cells obtained from Oryza sativa flowers were separated by one-dimensional polyacrylamide gel electrophoresis and globally identified by highly sensitive liquid chromatography coupled with tandem mass spectroscopy. Proteome analyses were also conducted for seedlings, callus, and pollen grains to compare their protein expression profiles to those of gametes. Database was searched using Mascot software (ver.2.2.1, Matrix Science, MA, USA) with the following parameters. The fixed modification was propionasmide (Cys) and variable modification parameters were pyro-Glu, acetylation (protein N-terminus), and oxidation (Met). The maximum missed cleavage was set at 3 with a peptide mass tolerance of +/– 15 ppm. Peptide charges from +2 to +4 states and MS/MS tolerances of +/– 0.8 Da were allowed.

2013-08-02 | PXD000265 | Pride

comparative cellular and transcriptomic analyses reveal differential gene networks regulating pollen development in synthetic haploid and tetraploid Brassica carinata

Project description:Purpose:Comparative cellular and transcriptome analyses was applied to characterize gene expression during male gametophytic development in Brassica carinata. Methods: floral buds (contain two developmental progress,1.1-1.6 mm and 1.8-6.5 long floral buds) were collected, then Separated male organs were kept in liquid nitrogen immediately until use. Total RNA was extracted using the TRIzol reagent (Invitrogen, Waltham, MA, USA). DNase (Promega, USA) was used to remove potential DNA contamination. For the quantitative real-time polymerase chain reaction (qRT-PCR) analysis Results: In this study, Up-regulated expression of DNA methylation probably affected pollen abortion in synthetic allohaploid B. carinata,and Down-regulated expression of cytokinin may affect pollen division and growth in synthetic allohaploid B. carinata Conclusions: Genes were shown male-preferred implies the dynamic changes of DNA methylation during the development of male gametes. The DEGs, related to CK signaling pathway and BR synthesis pathway were highly enriched in developmental male gametes, suggesting that CK played pivotal roles in male gamete development.

2023-01-06 | GSE185639 | GEO

Cell-type specific alternative splicing in the Arabidopsis germline

Project description:During sexual reproduction in flowering plants, the two haploid sperm cells embedded within the cytoplasm of a growing pollen tube are carried to the embryo sac for double fertilization. Pollen development in flowering plants is a dynamic process that encompasses changes at transcriptome and epigenome level. While the transcriptome of pollen and sperm cells in Arabidopsis thaliana is well documented, previous analyses were mostly based on expression at gene level. In-depth transcriptome analysis, particularly the extent of alternative splicing at the resolution of sperm cell and vegetative nucleus was still lacking. Therefore, we performed RNA-seq analysis to generate a spliceome map of Arabidopsis sperm cells and vegetative nuclei isolated from mature pollen grains. Based on our de-novo transcriptome assembly we identified 58039 transcripts, including 9681 novel transcripts, of which 2091were expressed in sperm cells and 3600 in vegetative nuclei. Our data from sperm cell and vegetative nucleus identified 468 genes that were regulated both at gene and splicing level, with many having functions in mRNA splicing, chromatin modification, and protein localization. Moreover, a comparison with egg cell RNA-seq data uncovered sex-specific regulation of transcription and splicing factors. Our study provides novel insights into a gamete specific alternative splicing landscape at unprecedented resolution.

2022-12-14 | GSE201115 | GEO

EVOREPRO: Maize RNA-seq data from pollen grain and pollen tube during the flowering development stage

Project description:Maize RNA-seq data from pollen grain, pollen tube, egg cell, sperm cell and zygots 12 and 24 hours after fertilization (HAF)

2021-04-14 | E-MTAB-9692 | biostudies-arrayexpress

Three MYB Transcription Factors Control Pollen Tube Differentiation Required for Sperm Release

2013-06-18 | GSE44405 | GEO

Three MYB Transcription Factors Control Pollen Tube Differentiation Required for Sperm Release

Project description:Flowering plants have immotile sperm that develop within pollen and must be carried to female gametes by a pollen tube. The pollen tube engages in molecular interactions with several cell types within the pistil and these interactions are essential for successful fertilization. We identified a group of three closely related pollen tube-expressed MYB transcription factors (MYB97, MYB101, MYB120), which are required for proper interaction of the pollen tube with the female gametophyte. These transcription factors are transcriptionally induced during growth in the pistil. They regulate a transcriptional network leading to proper differentiation and maturation of the pollen tube, promoting proper pollen tube-ovule interactions resulting in sperm release and double fertilization. We used microarrays to discover genes regulated by the transcription factors MYB97, MYB101 and MYB120 in pollen tubes growing through the pistil at 8 hours after pollination. Pistils were collected from ms1 (Male Sterile 1) pistils that were unpollinated, or pollinated with either wild type (Col-0) pollen or myb triple mutant (myb97-1, myb101-4, myb120-3) pollen for 8 hours. We sought to examine transcriptional changes that were taking place in pollen tubes before they reached ovules in wild type pollen tubes, and what portion of this transcriptional regulation was due to MYB97, MYB101 and MYB120. Analysis of growth in the pistil allows discovery of transcriptional changes taking place during pollen tube growth in its native environment, as opposed to mature pollen or in vitro grown pollen, which are essentially naive conditions, as neither have interacted with the pistil environment and any signalling factors found therein.

2013-06-18 | E-GEOD-44405 | biostudies-arrayexpress

Bovine sperm surface lectinome

Project description:Identification of bovine sperm surface carbohydrate-binding proteins relevant in two important aspects of fertilization: (i) formation of the sperm reservoir in the oviductal epithelium, and (ii) gamete recognition (oocyte-sperm interaction). Using whole sperm cells and a novel affinity capture method that combines proteolysis of protein-glycan complexes and mass spectrometry (i.e., CREDEX-MS), several lectins were enriched, identified by MS/MS proteomics, and mapped within the fertilization events in bovine species.

2016-04-28 | PXD003386 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data