Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression analysis of HEK293T cells after CHMP5 gene modulation

ABSTRACT: Microarray-based gene expression analysis identified genes differentially expressed in HEK293T cells after CHMP5 gene modulation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE17003 | GEO | 2010/07/08

SECONDARY ACCESSION(S): PRJNA117741

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene expression analysis of HEK293T cells after CHMP5 gene modulation

Project description:Microarray-based gene expression analysis identified genes differentially expressed in HEK293T cells after CHMP5 gene modulation. In this study, HEK293T cells, silenced for CHMP5 using sh-CHMP5 was compared to genome wide expression changes when compared to cellular transfections with a sh-luciferase control by Significance Analysis of Microarray data. Further, overexpression of CHMP5 using the vector pCMV_CHMP5 was compared to cellular transfections with an empty vector pCMV in the cell line HEK293T

2010-07-08 | E-GEOD-17003 | biostudies-arrayexpress

Network Analysis of HEK293T Gene Expression Profile Modulation During Nonviral Gene Delivery

Project description:The current understanding of the molecular factors underlying PEI-mediated transfection is largely unknown. The cationic polyethyleneimine (PEI) polymer gene delivery system was used to transfer the GFP gene to HEK293T cells. FACS separation of transfected (GFP positive), untransfected (GFP negative), and untreated cells enabled gene expression profiles to be obtained using Affymetrix® HG-U133A 2.0 microarrays for each cell population. Gene profiles were differentially compared for each population combination. Control (untreated), transfected (GFP positive), and untransfected (GFP negative) RNA samples were purified from HEK293T cells after FACS isolation. The experiment was repeated on triplicate days to run 3 microarrays for each cell population. Only 2 untreated replicates are included in this submission.

2012-06-02 | E-GEOD-38422 | biostudies-arrayexpress

Gene expression analysis of JVM-2 and Granta-519 after CCDC50 gene modulation

Project description:Microarray-based gene expression analysis identified genes differentially expressed in JVM-2 and Granta-519 after CCDC50 gene modulation.

2010-06-03 | GSE16435 | GEO

Network Analysis of HEK293T Gene Expression Profile Modulation During Nonviral Gene Delivery

2012-06-03 | GSE38422 | GEO

Basal Gene Expression Measurements in HEK293T Cells

Project description:Gene expression microarray profile for human embryonic kidney cells (HEK293T, CRL-11268) under untreated conditions.

2010-03-31 | GSE21092 | GEO

Basal Gene Expression Measurements in HEK293T Cells

Project description:Gene expression microarray profile for human embryonic kidney cells (HEK293T, CRL-11268) under untreated conditions. Gene expression microarray profile for human embryonic kidney cells (HEK293T, CRL-11268) under untreated conditions (DMEM, 10% FBS).

2010-04-12 | E-GEOD-21092 | biostudies-arrayexpress

Gene expression profiles of HEK293T cells following EBV-miR-BART6-3p mimics transfection

Project description:EBV encodes 44 mature miRNAs, a number of which have been found to promote carcinogenesis by targeting host genes. To determine the biological functions of the BART cluster miRNAs, we singly transfected these miRNAs into HEK293T cells. Interestingly, we found that overexpression of EBV-miR-BART6-3p in HEK293T cells dramatically altered HEK293T cell shape. To explore the mechanism of EBV-miR-BART6-3p-mediated inhibition of cell migration and invasion, we attempted to identify its downstream host cellular genes by whole genome microarray, which contains probes to all known human protein coding genes (mRNAs) and long non-coding RNA genes (lncRNAs).

2017-02-09 | GSE81528 | GEO

SRSF1 knockdown in HEK293T cells with ionizing radiation

Project description:The goal of the study is to identify differentially expressed isoforms in response to SRSF1 knockdown and/or ionizing radiation in HEK293T cells

2024-09-30 | GSE242550 | GEO

Gene expression changes in HEK293T cells upon overexpression of ING1b and GADD45a

Project description:ING1b and GADD45a are nuclear proteins involved in the regulation of cell growth, apoptosis and DNA repair. We found that ING1b and GADD45a physically and functionally interact in the epigenetic regulation of specific target genes. In order to characterise the functional ING1b-GADD45a interaction, we performed a gain-of-function experiment in HEK293T cells by individual and combinatorial plasmid transfections and then analysed the transcriptional response via expression microarray profiling. HEK293T cells were transiently transfected with expression plasmids encoding human GADD45a and/or human ING1b (full-length or without its PHD-domain) and harvested 48h post-transfection for Illumina microarray profiling. Two independently transfected replicate samples of each condition were analysed. Empty vector (control) transfections served as reference samples.

2013-02-04 | E-GEOD-43317 | biostudies-arrayexpress