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An optimized RNA amplification method for prokaryotic expression profiling analysis

ABSTRACT: Conventional prokaryotic RNA labeling method usually requires large amounts of starting materials and tends to generate high background signals. Recently, two novel methods based on amplification systems were introduced. Here, we compared three alternative strategies: direct labeling method, ployadenylation-involved oligo-dT priming amplification method and random priming amplification method (hereafter referred to as DL, PAOD and RPA method in this article) for prokaryotic RNA labeling employing the expression profiling investigation in Escherichia coli (E. coli) heat shock model.

ORGANISM(S): Escherichia coli

PROVIDER: GSE17074 | GEO | 2009/07/25

SECONDARY ACCESSION(S): PRJNA119813

REPOSITORIES: GEO

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An optimized RNA amplification method for prokaryotic expression profiling analysis

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