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Topoisomerase 1 activity during mitotic transcription favors the transition from mitosis to G1 [ChIP-seq]

ABSTRACT: By following chromatin occupancy of RNAPII and TOP1 using ChIP-seq throughout mitosis, we found that TOP1 is required for RNAPII translocation along genes. The stimulation of TOP1 activity through its interaction with RNAPII during elongation, allowed RNAPII clearance from genes in prometaphase and enabled proper chromosomal segregation. Interference with the TOP1-RNAPII interaction or acute depletion of TOP1 at the onset of mitosis impaired RNAPII spiking at promoters, which is necessary for the first pioneering round of transcription during mitotic exit, and triggered defects in the transcriptional program of the post-mitotic cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE171132 | GEO | 2021/11/11

REPOSITORIES: GEO

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