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Genome-wide mapping of histone H3 Lysine 4 trimethylation (H3K4me3) and its Involvement in fatty acid biosynthesis in sunflower developing seeds.

ABSTRACT: Histone modifications are of paramount importance during plant development. Investigating chromatin remodeling in developing oilseeds sheds light on the molecular mechanisms controlling fatty acid metabolism as well as facilitates the identification of new functional regions in oil crop genomes. The present study characterized the epigenetic modifications H3K4me3 in relationship with the expression of fatty acid related-genes and transcription factors in developing sunflower seeds.

ORGANISM(S): Helianthus annuus

PROVIDER: GSE171309 | GEO | 2021/04/28

REPOSITORIES: GEO

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Analysis of the coding and non-coding RNA temporal transcriptome of developing seeds in sunflower [ncRNA-seq]

Project description:miRNA-sequencing of seeds in three developmental stages was performed to reveals gene regulation in relation to oil and fatty acid accumulation in sunflower

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Analysis of the coding and non-coding RNA temporal transcriptome of developing seeds in sunflower [RNA-seq]

Project description:RNA-sequencing (lncRNA,mRNA and circRNA) of seeds in three developmental stages was performed to reveals gene regulation in relation to oil and fatty acid accumulation in sunflower

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Genome-wide mapping of histone H3 Lysine 4 trimethylation (H3K4me3) and its Involvement in fatty acid biosynthesis in sunflower developing seeds.

Project description:Genome-wide mapping of histone H3 Lysine 4 trimethylation (H3K4me3) and its Involvement in fatty acid biosynthesis in sunflower developing seeds.

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Identification of ABA regulated genes in sunflower

Project description:Abiotic stress and more specifically drought is the major limiting factor for sunflower production. ABA is a key hormone for drought stress response in plants and sunflower. This experiment aims at identifying ABA responsive pathways in order to better understand sunflower responses to drought. We studied in parallel microRNA profiles on the same samples and we will try to identify sunflower microRNA regulated genes in response to ABA. The ultimate goal will be improve sunflower breeding through selection of key drought response genes.-The experiment consisted of 3 repeats of four 12-day-old-plantlets of sunflower genotype SF193 (INRA code: XRQ) grown in growth chamber conditions and submitted to a 6-hour-treatment of 10 µM absissic acid or not. Growth conditions were 14h light at 23°C and 10h night at 20°C under fluorescent bulbs. Plants were grown in 6 hydroponic boxes containing 20 litres of aerated liquid culture medium (as described in Massonneau et al., 2001 Planta). Leaves (not cotyledons) 1 to 4 were harvested 4 hours after light onset and frozen immediately in liquid nitrogen.

2012-12-09 | GSE22519 | GEO

Identification of ABA regulated genes in sunflower

Project description:Abiotic stress and more specifically drought is the major limiting factor for sunflower production. ABA is a key hormone for drought stress response in plants and sunflower. This experiment aims at identifying ABA responsive pathways in order to better understand sunflower responses to drought. We studied in parallel microRNA profiles on the same samples and we will try to identify sunflower microRNA regulated genes in response to ABA. The ultimate goal will be improve sunflower breeding through selection of key drought response genes.-The experiment consisted of 3 repeats of four 12-day-old-plantlets of sunflower genotype SF193 (INRA code: XRQ) grown in growth chamber conditions and submitted to a 6-hour-treatment of 10 ÂµM absissic acid or not. Growth conditions were 14h light at 23Â°C and 10h night at 20Â°C under fluorescent bulbs. Plants were grown in 6 hydroponic boxes containing 20 litres of aerated liquid culture medium (as described in Massonneau et al., 2001 Planta). Leaves (not cotyledons) 1 to 4 were harvested 4 hours after light onset and frozen immediately in liquid nitrogen. 6 arrays - SUNFLOWER; treated vs untreated comparison

2012-12-09 | E-GEOD-22519 | biostudies-arrayexpress

Genome-Wide Mapping of Histone H3 Lysine 4 Trimethylation (H3K4me3) and Its Involvement in Fatty Acid Biosynthesis in Sunflower Developing Seeds.

Project description:Histone modifications are of paramount importance during plant development. Investigating chromatin remodeling in developing oilseeds sheds light on the molecular mechanisms controlling fatty acid metabolism and facilitates the identification of new functional regions in oil crop genomes. The present study characterizes the epigenetic modifications H3K4me3 in relationship with the expression of fatty acid-related genes and transcription factors in developing sunflower seeds. Two master transcriptional regulators identified in this analysis, VIV1 (homologous to Arabidopsis ABI3) and FUS3, cooperate in the regulation of WRINKLED 1, a transcriptional factor regulating glycolysis, and fatty acid synthesis in developing oilseeds.

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RNASeq of Developing Arabidopsis Seeds Producing Hydroxy-Fatty Acids

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RNASeq of Developing Arabidopsis Seeds Producing Hydroxy-Fatty Acids

2014-01-10 | GSE53952 | GEO

eqtl-Identification of expression QTL of ABA response in sunflower

Project description:affy_eqtl_sunflower - eqtl - Abiotic stress and more specifically drought is the major limiting factor for sunflower production. ABA is a key hormone for drought stress response in plants and sunflower. This experiment aims at identifying ABA responsive pathways in order to better understand sunflower responses to drought. The ultimate goal will be improve sunflower breeding through selection of key drought response genes. The experiment consisted of 1 or 2 (and 3 for the sample 209) repeats of 113 recombinant imbred lines and 11 repeats of the population parents SF1935 (INRA code XRQ), 11 SF326 (INRA code: PSC8) and the F1 hybrid 10 INEDI. The genotypes were grown in growth chamber conditions and submitted to a 6-hour-treatment of 0.5 ÂµM absissic acid or not. Growth conditions were 14h light at 23Â°C and 10h night at 18Â°C under fluorescent bulbs. Plants were grown in 18 hydroponic boxes (9 for ABA treatment, 9 for control) containing 20 litres of aerated liquid culture medium (as described in Massonneau et al., 2001 Planta). Leaves (not cotyledons) from the 15-day-old-plantlets of sunflower were harvested 6 hours after treatment; the three repeats were pooled for each genotype and frozen immediately in liquid nitrogen. 236 arrays - SUNFLOWER; treated vs untreated comparison Grp suffixes such as -A, -AA, and bis, are there to differentiate the same replicates.

2013-06-12 | E-GEOD-38812 | biostudies-arrayexpress

eqtl-Identification of expression QTL of ABA response in sunflower

Project description:affy_eqtl_sunflower - eqtl - Abiotic stress and more specifically drought is the major limiting factor for sunflower production. ABA is a key hormone for drought stress response in plants and sunflower. This experiment aims at identifying ABA responsive pathways in order to better understand sunflower responses to drought. The ultimate goal will be improve sunflower breeding through selection of key drought response genes. The experiment consisted of 1 or 2 (and 3 for the sample 209) repeats of 113 recombinant imbred lines and 11 repeats of the population parents SF1935 (INRA code XRQ), 11 SF326 (INRA code: PSC8) and the F1 hybrid 10 INEDI. The genotypes were grown in growth chamber conditions and submitted to a 6-hour-treatment of 0.5 µM absissic acid or not. Growth conditions were 14h light at 23°C and 10h night at 18°C under fluorescent bulbs. Plants were grown in 18 hydroponic boxes (9 for ABA treatment, 9 for control) containing 20 litres of aerated liquid culture medium (as described in Massonneau et al., 2001 Planta). Leaves (not cotyledons) from the 15-day-old-plantlets of sunflower were harvested 6 hours after treatment; the three repeats were pooled for each genotype and frozen immediately in liquid nitrogen.

2013-06-12 | GSE38812 | GEO

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