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Transcriptional adaptation to DNA replication stress induced by CCNE1 overexpression

ABSTRACT: We overproduced human cyclin E from a stably-integrated inducible CCNE1 transgene in RPE1-hTERT cells over a period of weeks. CCNE1 overproduction induced replication stress, then slow proliferation, followed by adaptation to normal growth phenotype. We analyzed mRNA transcripts at key points of the timecourse: control, 2 days, 15-18 days, 39-51 days, and at a point several weeks after induction was withdrawn

ORGANISM(S): Homo sapiens

PROVIDER: GSE171845 | GEO | 2021/10/01

REPOSITORIES: GEO

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