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Next Generation Sequencing of Wild Type (Col-0), fio1-1 and fio1-2 methylated RNA immunoprecipitation sequencing in Arabidopsis thaliana

ABSTRACT: Plants were grown on 1/2 MS for 14 days under LD conditions and RNA was extracted and sequenced

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE171924 | GEO | 2022/08/24

REPOSITORIES: GEO

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Next Generation Sequencing of Wild Type (Col-0) , fio1-1 and fio1-2 Transcriptomes in Arabidopsis thaliana

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Next Generation Sequencing of Wild Type (Col-0), fio1-1 and fio1-2 methylated RNA immunoprecipitation and transcriptome in Arabidopsis thaliana

Project description:This SuperSeries is composed of the SubSeries listed below.

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Next Generation Protein Sequencing

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Mapping QTLs for 15 morpho-metric traits in <i>Arabidopsis thaliana</i> using Col-0 × Don-0 population.

Project description:Genome wide quantitative trait loci (QTL) mapping was conducted in Arabidopsis thaliana using F2 mapping population (Col-0 × Don-0) and SNPs markers. A total of five linkage groups were obtained with number of SNPs varying from 45 to 59 per linkage group. The composite interval mapping detected a total of 36 QTLs for 15 traits and the number of QTLs ranged from one (root length, root dry biomass, cauline leaf width, number of internodes and internode distance) to seven (for bolting days). The range of phenotypic variance explained (PVE) and logarithm of the odds ratio of these 36 QTLs was found be 0.19-38.17% and 3.0-6.26 respectively. Further, the epistatic interaction detected one main effect QTL and four epistatic QTLs. Five major QTLs viz. Qbd.nbri.4.3, Qfd.nbri.4.2, Qrdm.nbri.5.1, Qncl.nbri.2.2, Qtd.nbri.4.1 with PVE > 15.0% might be useful for fine mapping to identify genes associated with respective traits, and also for development of specialized population through marker assisted selection. The identification of additive and dominant effect QTLs and desirable alleles of each of above mentioned traits would also be important for future research.

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Two distinct extracellular RNA signatures released by a single cell type identified by microarray and next-generation sequencing [miRNA]

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Two distinct extracellular RNA signatures released by a single cell type identified by microarray and next-generation sequencing [total RNA]

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