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Transcriptome analysis of iron and phosphorus interaction in rice seedlings

ABSTRACT: The antagonistic interaction between iron (Fe) and phosphorus (P) has been noted in the area of plant nutrition. To understand the physiology and molecular mechanisms of this interaction, we studied the growth performance, nutrient concentration, and gene expression profiles of root and shoot segments derived from 10-d-old rice (Oryza sativa) seedlings under four different nutrient conditions: (1) full strength of Fe and P (+Fe+P); (2) full strength of P and no Fe (-Fe+P); (3) full strength of Fe and no P (+Fe-P); and (4) without both Fe and P (-Fe-P). We used Affymetrix genechip to detail the global programme of gene expression underlying different Fe and P supply conditions identified distinct classes of up-regulated genes.

ORGANISM(S): Oryza sativa

PROVIDER: GSE17245 | GEO | 2009/07/22

SECONDARY ACCESSION(S): PRJNA118951

REPOSITORIES: GEO

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Transcription profiling of rice seedlings iron and phosphorus interaction in

Project description:The antagonistic interaction between iron (Fe) and phosphorus (P) has been noted in the area of plant nutrition. To understand the physiology and molecular mechanisms of this interaction, we studied the growth performance, nutrient concentration, and gene expression profiles of root and shoot segments derived from 10-d-old rice (Oryza sativa) seedlings under four different nutrient conditions: (1) full strength of Fe and P (+Fe+P); (2) full strength of P and no Fe (-Fe+P); (3) full strength of Fe and no P (+Fe-P); and (4) without both Fe and P (-Fe-P). We used Affymetrix genechip to detail the global programme of gene expression underlying different Fe and P supply conditions identified distinct classes of up-regulated genes. Experiment Overall Design: The GeneChip analysis experiment was designed as a two-factor experiment with four treatments: (1) +P+Fe; (2) âFe+P; (3) +FeâP; (4) âFeâP. The experiment included two biological replicates. Each sample represented root and shoot material from five to 10 plants.

2009-08-07 | E-GEOD-17245 | biostudies-arrayexpress

Phosphorus deficiency alleviates cyanobacterial iron-limitation through a direct PhoB_x0002_ mediated gene regulation

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Comparative transcriptomic analysis of iron deficiency response in roots of Oryza sativa and O. rufipogon

Project description:Iron (Fe) is an essential micronutrient for almost all organisms. However, Fe is frequently inaccessible to plants. To acquire insoluble Fe from soil, rice (Oryza sativa L.) plants employ a Combined Strategy, which is composed by all features of Strategy II, common to all Poaceae species, and some features of the Strategy I from non-Poaceae. To understand the evolution of Fe uptake mechanisms, we analyzed the root transcriptomic response to Fe deficiency in two species from the Oryza genus: O. sativa and its wild progenitor O. rufipogon. A total of 622 and 2,017 genes were found to be differentially expressed in O. sativa and O. rufipogon, respectively. Among the genes regulated in both species, we found the Fe transporters associated with Strategy I, such as IRT1, IRT2 and NRAMP1; and genes associated with Strategy II, such as YSL15 and IRO2.

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Genotype variation in rice (Oryza sativa L.) tolerance to Fe toxicity might be linked to root cell wall lignification

Project description:Iron (Fe) is an essential element to plants, but can be harmful if accumulated to toxic concentrations. Fe toxicity can be a major nutritional disorder in rice (Oryza sativa) when cultivated under waterlogged conditions, as a result of excessive Fe solubilization of in the soil. However, little is known about the basis of Fe toxicity and tolerance at both physiological and molecular level. Here we aim at understand the genotypic differences in two rice cultivars with contrasting phenotypes under Fe toxicity.

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Project description:OsPI1 was isolated from rice and revealed to belong to the TPSI1/Mt4 family, a phosphorus deficiency responsive gene family. The importance of this family gene for phosphorus deficiency responses of plants had been already reported, though its actual role is still unclear. To understand the plants response mechanisms for phosphorus deficiency and the role of OsPI1 in it, we constructed a OsPI1 RNAi line (ospi1-2) for this study. We used the 22K rice Oligoarray from Agilent technologies to study the transcript profile in the shoots and roots of ospi1-2 and Oryza sativa L. cv. Kitaake wild type (WT) rice plants under constitutive and phosphorus deficiency at seedling stage. Keywords: Influences of OsPI1 knockdown

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