Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq analysis of the gene expression profile regulated by CHIR and Spautin-1 in mouse ESCs

ABSTRACT: Purpose:The goals of this study is to analyze the gene expession pattern in mouse embryonic stem cells (ESCs) treated with CHIR99021(CHIR) in the presence or absence of Spautin-1 for 24 h.

ORGANISM(S): Mus musculus

PROVIDER: GSE172469 | GEO | 2021/04/21

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

RNA-seq analysis of the gene expression profile regulated by Gadd45g in mouse ESCs

Project description:Purpose: The goals of this study is to analyze the gene expession pattern in mouse embryonic stem cells (ESCs) ovexpressed flag or mouse flag tagged gadd45g

2021-04-22 | GSE172474 | GEO

RNA-seq analysis of the gene expression profile regulated by Prdm14 in mouse ESCs

Project description:Purpose:The goals of this study is to analyze the gene expession pattern in mouse embryonic stem cells (ESCs) ovexpressed flag or mouse flag tagged Prdm14

2021-10-01 | GSE184802 | GEO

Next generation sequencing facilitates lineage identity of RA-induced and RA/CHIR-induced ESCs

Project description:To uncover the mechanism that Wnt signaling modulates RA-induced ESC differentiation, we conducted time course RNA-seq analysis during the differentiation. Transcriptome profiles of RA-induced and RA/CHIR-induced ESC differentiation were generated by deep sequencing, in triplicate, using Illumina HiSeq 2000. The RNA-seq data revealed a significant decrease in mesoderm (one of the major source of vascular SMCs) markers and increase in PrE markers in CHIR treated samples, while other lineage markers showed no significant expression, implying a diverging to PrE fate by Wnt activation. Subsequent hierarchical clustering analysis also showed that the gene expression profiles of cells derived from RA induction alone clustered with adult SMCs, while cells derived from RA/CHIR induction gradually clustered to XEN cells with differentiation and was very close to XEN cells at day 16, suggesting that RA/CHIR-induced ESCs differentiated into PrE lineage. This study provided new insights into the importance of RA/Wnt crosstalk in the lineage differentiation of ESCs.

2019-12-10 | GSE123632 | GEO

RNA-seq analysis of the gene expression profile regulated by CHIR and Spautin-1 in mouse ESCs

Project description:RNA-seq analysis of the gene expression profile regulated by CHIR and Spautin-1 in mouse ESCs

| PRJNA723353 | ENA

Global gene expression profile of mouse ESC-2i, EpiSC-CHIR/XAV and EpiSC-FGF2/activin

Project description:The requirements for self-renewal differ between EpiSCs and ES cells and the underlying mechanism is largely unknown. Here we show that mouse EpiSCs can be efficiently derived and robustly propagated even from single cells, using two small-molecule inhibitors: CHIR99021 and XAV939. The whole-genome microarray analyses is performed to confirm the identity of EpiSC maintained in CHIR/XAV by comparing the expression profile in EpiSC-CHIR/XAV to those in ESC maintained in 2i and EpiSC maintained in FGF2/activin. Total RNA from ESC-2i, EpiSC-CHIR/XAV, and EpiSC-FGF2/activin were extracted for microarray analisys

2011-08-18 | E-GEOD-31461 | biostudies-arrayexpress

Global gene expression profile of mouse ESC-2i, EpiSC-CHIR/XAV and EpiSC-FGF2/activin

2011-08-19 | GSE31461 | GEO

Next generation sequencing facilitates lineage identity of RA-induced and RA/CHIR-induced ESCs

Project description:Next generation sequencing facilitates lineage identity of RA-induced and RA/CHIR-induced ESCs

| PRJNA509431 | ENA

E. coli gene expression following CHIR-090 treatment

2009-10-20 | GSE18623 | GEO

Transcription profiling of E. coli treated with CHIR-090, an inhibitor of lipid A biosythesis

Project description:CHIR-090 is an inhibitor of lipid A biosytnesis. CHIR-090 treatment on E. coli sinificantly increased the expression of fatty acid biosythesis gene fabA and fabB. Experiment Overall Design: The lipid A biosynthesis in E. coli was inhibited by CHIR-090, and the RNA was extracted after 10 minutes treatment to capture the effect of increased saturated fatty acid on global gene expression.

2009-10-27 | E-GEOD-18623 | biostudies-arrayexpress

Identify the downstream targets of CHIR99021 and XAV939 in EpiSC using microarray analysis

Project description:The requirements for self-renewal differ between EpiSCs and ES cells and the underlying mechanism is largely unknown. Here we show that mouse EpiSCs can be efficiently derived and robustly propagated even from single cells, using two small-molecule inhibitors: CHIR99021 and XAV939. To better define how CHIR and XAV act together to maintain EpiSC self-renewal, we performed microarray analyses to identify their downstream targets. The data show the genes regulated by addition of CHIR, XAV or both. EpiSCs starved in serum free growth medium (shown in growth protocol) for 12hrs were treated with CHIR (2hrs), XAV(4hrs), or both, after which total RNA was extracted for analysis.

2011-08-24 | E-GEOD-31544 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data