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Acute detachment of hexokinase II from mitochondria modestly increases oxygen consumption of the intact mouse heart

ABSTRACT: Cardiac hexokinase II (HKII) can translocate between cytosol and mitochondria and change its cellular expression with pathologies such as ischemia–reperfusion, diabetes and heart failure. The cardiac metabolic consequences of these changes are unknown. Here we measured energy substrate utilization in cytosol and mitochondria using stabile isotopes and oxygen consumption of the intact perfused heart for 1) an acute decrease in mitochondrial HKII (mtHKII), and 2) a chronic decrease in total cellular HKII.

ORGANISM(S): Mus musculus

PROVIDER: GSE173341 | GEO | 2021/04/27

REPOSITORIES: GEO

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