Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MRNA-seq analysis of Caenorhabditis elegans starved L1 larvae with and without AMA-1/Pol II degradation in the soma and germ line during L1 arrest

ABSTRACT: Whole starved L1 larvae were collected with and without AMA-1 degradation by the auxin-inducible degron (AID) system in the soma and germ line between 36 and 132 hours of starvation along with controls.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE173655 | GEO | 2022/10/11

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

mRNA-seq analysis of Caenorhabditis elegans starved L1 larvae with and without AMA-1/Pol II degradation in the soma and germ line during L1 arrest

Project description:mRNA-seq analysis of Caenorhabditis elegans starved L1 larvae with and without AMA-1/Pol II degradation in the soma and germ line during L1 arrest

| PRJNA726549 | ENA

Starved HEK293 cells

Project description:Starved HEK293 cells

| PRJNA533129 | ENA

mRNA-seq time series of Caenorhabditis elegans L1 starvation

Project description:Whole starved L1 larvae were collected at twelve time points spanning from hatching to death.

2022-10-11 | GSE173656 | GEO

mRNA-seq of L1 larvae recovering from arrest

2012-04-25 | GSE33023 | GEO

Using Auxin-inducible-degradation System to Interrogate Tissue-specific Transcriptional Programs of HSF-1 in Lifespan Assurance [HSF-1 transcriptome in the soma]

Project description:To determine the transcriptional program of HSF-1 in lifespan assurance in C. elegans, we coupled HSF-1 depletion specifically from the soma by auxin-inducible degron (AID) with RNA-seq analyses in whole animals. Depletion of HSF-1 from the soma was performed by transferring worms that express the E3 ligase TIR1 in somatic tissues and carry AID insertion to the endogenous HSF-1 onto NGM plates containing 1mM auxin (indole- 3-acetic acid, Sigma). The mock treatment was done by transferring worms to plates only containing the vehicle ethanol (EtOH). We analyzed the transcriptomic changes by RNA-seq upon soma-specific depletion of HSF-1 initiated at young adult stage for different length of time. We included the strains that only express TIR1 but do not have degron insertion at HSF-1 (CA1200) to control for the effects of auxin treatment and AID insertion into HSF-1. We performed analyses in the wild-type background (JTL611) as well as in the longlived, germline stem cell (GSC) arrested glp-1(e2141) mutant (JTL667). The AID model in fem-3(q20) mutant (JTL670) was included as a control for JTL667. JTL670 (fem-3) is sterile at 25°C as JTL667 (glp-1) but has normal lifespan.

2022-05-19 | GSE203087 | GEO

Drosophila TRAP soma vs germline

Project description:Drosophila TRAP soma vs germline

| PRJNA961150 | ENA

mRNA-seq analysis of Caenorhabditis elegans N2, daf-2(e1370), irld-39(duk1);irld-52(duk17), and daf-2(e1370); irld-39(duk1); irld-52(duk17) starved L1 larvae

Project description:Whole starved L1 larvae from four genotypes (N2, daf-2(e1370), irld-39(duk1);irld-52(duk17), and daf-2(e1370); irld-39(duk1); irld-52(duk17)) were collected after 12 hours of starvation

2023-01-06 | GSE174319 | GEO

mRNA-seq of L1 larvae recovering from arrest

Project description:We used RNA-seq to sequence the transcriptome of worms arrested in the first larval stage after hatching without food and starved for 12 hours, then a timecourse of animals recovering from arrest after feeding. We collected samples after 12 hours of starvation and 1, 3 and 6 hours after feeding. Four time points (12 hr starvation and 1, 3, and 6 hr of recovery) with three biological replicates at times 0 and 6 and two biological replicates at times 1 and 3.

2012-04-25 | E-GEOD-33023 | biostudies-arrayexpress

Transcriptome of wild type N2 worms at embronic stage and starved L1 stage

Project description:Transcription factors are key components of regulatory networks that control development, as well as the response to environmental stimuli. We have established an experimental pipeline in C. elegans that permits global identification of the binding sites for transcription factors using chromatin immunoprecipitation and deep sequencing. We describe and validate this strategy, and apply it to the transcription factor PHA-4, which plays critical roles in organ development and starvation stress response. We defined binding sites for PHA-4 during formation of the embryonic pharynx, and in starved larvae. PHA-4 binds to thousands of locations throughout the genome. These sites shift dramatically between embryos and larvae, from developmentally regulated genes to genes involved in metabolism. We acquired transcriptome of wild type N2 worms at embyonic and starved L1 stages using RNA-seq method in comparing to the ChIP-seq experiment to study transcription factor binding. The wild type worms were cultured the same as the transgenic worms for transcription factor binding experiment. Keywords: Transcriptome Analysis For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Two development stages embryo and starved larvae each contains 3 technical replicates are reported

2009-06-11 | E-GEOD-16552 | biostudies-arrayexpress

Transcriptome of wild type N2 worms at embronic stage and starved L1 stage

2009-06-11 | GSE16552 | GEO