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Comparison of Clinical Staphylococcus aureus Strains Demonstrating Differing Susceptibilities to the Biocide Ethanol

ABSTRACT: Ethanol is utilized widely in medicine for both its antiseptic and disinfectant capabilities. In an effort to understand the effects of ethanol on a very common human pathogen, we have determined the ethanol-stimulon of two well characterized unrelated clinical S. aureus methicillin resistant strains LP9 and MM66 with ethanol minimum inhibitory concentrations (MIC) of 10 and 7% respectively. Using S. aureus microarrays and array data analysis protocols (NIAID's Pathogen Functional Genomics Resource Center) we have characterized the transcriptomes of LP9 and MM66 following 15 min exposure to 10% ethanol. Induction with 10 % ethanol led to the altered regulation of 797 genes in LP9 and 899 genes in MM66. There was an overlap of 600 commonly altered genes in both strains and of these, SAMMD identified 50 genes that are uniquely altered only in the ethanol stimulon compared to the other 93 transcriptomes used for comparison. SAMMD analysis further revealed that 259 of the 600 (43%) commonly Eth-altered genes, overlapped with the stringent response induced with the antibiotic mupirocin. Interestingly the gene SACOL2518 up-regulated 20.4 and 23 fold in LP9 and MM66 respectively has a RelA- and SpoT- like domain. We have also found the MICs and MBCs for both LP9 and MM66 to be affected by the addition of certain amino acids. Genes involved with the heat shock response were also among some of the highest similarly up-regulated genes. Overall, SAMMD found 30% (43 genes) in common between the heat shock and ethanol transcriptomes of LP9 and MM66. clpB encoding the clpB chaperone was the highest induced gene in both strains. groES, groEL, hrcA, grpE and dnaK were also some of the highest up-regulated genes in both strains.

ORGANISM(S): Staphylococcus aureus

PROVIDER: GSE17391 | GEO | 2009/08/17

SECONDARY ACCESSION(S): PRJNA118829

REPOSITORIES: GEO

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Comparison of Clinical Staphylococcus aureus Strains Demonstrating Differing Susceptibilities to the Biocide Ethanol

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