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Bulk RNA sequencing of acetylcholine-synthesizing macrophages from subcutaneous adipose tissue

ABSTRACT: We aimed to study the transcriptomic profile of acetylcholine-synthesizing adipose macrophages. Using a ChAT-eGFP reporter mouse (ChAT, or choline acetyltransferase, is the main enzyme for acetylcholine biosynthesis) acutely exposed to cold temperature, we used FACS to isolate ChAT-eGFP+ and ChAT-eGFP- macrophages from inguinal subcutaneous white adipose tissue (IWAT). We subjected these cells to bulk RNA-seq to better understand the transcriptomic landscape of cholinergic macrophages under cold stress and their role in beige fat thermogenesis.

ORGANISM(S): Mus musculus

PROVIDER: GSE174345 | GEO | 2021/07/29

REPOSITORIES: GEO

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