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Single-cell deconvolution of colon organoids exposed to carcinogens reveals novel insights into the early molecular events defining the role of smoking in colorectal cancer

ABSTRACT: Here, we used bulk RNA-seq data derived from healthy colon organoids (un)exposed to carcinogen. Through the use of external single cell RNA-seq data, we estimate changes in cell composition. We extend this analysis by controlling for cell composition and performing WGCNA to identify modules of co-expression differentially affected by carcinogen treatment in colon organoids.

ORGANISM(S): Homo sapiens

PROVIDER: GSE174650 | GEO | 2021/09/22

REPOSITORIES: GEO

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