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High-resolution transciptome mapping and N6-methyladenosine modification mapping of RAW264.7 cell lines treated with TCP, TBP or TIP.

ABSTRACT: Purpose:Detect transcriptome-wide mapping of m6A modifications by MeRIP-seq between normal and chemical-treated RAW264.7 cells. Methods:Total RNA was extracted using TRIzol Reagent followed by chemical fragmentation. The fragmented RNA was incubated with anti-m6A antibody. The desired m6A-enriched RNA eluate was used to construct cDNA library in parallel with input control. Conclusion: Our study revealed the difference of m6A-methylated RNA between normal and TCP/TBP/TIP-treated cells by RNA-seq and MeRIP-seq technologies.

ORGANISM(S): Mus musculus

PROVIDER: GSE174678 | GEO | 2021/07/01

REPOSITORIES: GEO

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